[Effects of botulinum toxin type A on the expression of alpha-SMA and myosin-II of fibroblasts in scars].

Abstract

OBJECTIVE:

To investigate the effects of botulinum toxin type A (BTXA) on the expression of alpha smooth muscle actin(alpha-SMA) and myosin-II of fibroblasts in scars. Methods Fibroblasts were isolated from tissue specimens of scars contracture. Cells from passages 3-5 were randomly divided into 3 groups (control group, low BTXA group (1 U/10(6) Cells), and high BTXA group (2.5 U/ 10(6)Cells)). Growth condition of fibroblasts was observed at 1 , 4, 7 day after BTXA treated. Changes of alpha-SMA and myosin-II in fibroblasts were detected by Western blot.

RESULTS:

Fibroblasts grew well in control group. The proliferation was decreased 4 days later in BTXA groups. Lots of apoptotic cells were seen in high BTXA group at 7th day. Proteins of alpha-SMA and myosin-II in fibroblasts were statistically different between BTXA group and control groups at 4th day (P < 0.05). The expression of alpha-SMA and myosin-II in low BTXA group was higher than that in high BTXA group at 7th day (P < 0.05).

CONCLUSIONS:

BTXA could induce the apoptosis of fibroblasts and decrease the expression of alpha-SMA and myosin-II in fibroblasts. The inhibitory effect was strengthened with BTXA concentration increase within a certain range.

• Authors: Tongtong Yan, Minliang Chen, Kui Ma, Liming Liang, Chang Liu, Linying Lai, Xiaobing Fu
• Journal: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery (Zhonghua Zheng Xing Wai Ke Za Zhi) Vol. 30 Issue 2 Pg. 118-21 (Mar 2014) ISSN: 1009-4598 [Print] China
• PMID: 24941763 (Publication Type: Journal Article)

Chemical References

• Actins
• Botulinum Toxins, Type A
• Myosin Type II

Topics

• Actins (metabolism)
• Botulinum Toxins, Type A (pharmacology)
• Cicatrix
• Fibroblasts (drug effects, metabolism)
• Humans
• Muscle, Smooth (metabolism)
• Myosin Type II (metabolism)
• Random Allocation