Preparation of immunostimulatory single-walled carbon nanotube/CpG DNA complexes and evaluation of their potential in cancer immunotherapy.

Abstract

Carbon nanotubes (CNTs) have many interesting properties. In particular, their photohyperthermic effect by near-infrared (NIR) irradiation could be used to kill cancer cells, and could thus be applied in photohyperthermic therapy. However, the solubility of CNTs must be improved before they can be used in biological applications. As DNA is reported to disperse the CNTs in aqueous solution with π-π interactions, we hypothesis that immunostimulatory CpG DNA may also disperse the CNTs in aqueous solution. In this study, we used CpG DNA to disperse single-walled CNTs (SWCNTs) in aqueous solution, in order to combine photohyperthermic effect and immunoactivation together to achieve a more effective cancer therapy. As expected, CpG DNA effectively dispersed the SWCNTs in aqueous solution via the formation of SWCNT/CpG DNA complexes. Moreover, the immunoreactivity of the SWCNT/CpG DNA complexes was investigated. The results showed that intratumoral administration of the SWCNT/CpG DNA complexes in mice enhanced the production level of inflammatory cytokines in tumor tissues. Finally, we evaluated the antitumor effects of the SWCNT/CpG DNA complexes in tumor-bearing mice. The result indicated that intratumoral administration of the SWCNT/CpG DNA complexes combined with NIR irradiation was a more effective approach to prevent the proliferation of tumor growth.

• Authors: Shuwen Zhou, Yasuhiko Hashida, Shigeru Kawakami, Junya Mihara, Tomokazu Umeyama, Hiroshi Imahori, Tatsuya Murakami, Fumiyoshi Yamashita, Mitsuru Hashida
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 471 Issue 1-2 Pg. 214-23 (Aug 25 2014) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 24861942 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• CPG-oligonucleotide
• Cytokines
• Drug Carriers
• Nanotubes, Carbon
• Oligodeoxyribonucleotides

Topics

• Adjuvants, Immunologic (administration & dosage, pharmacology, therapeutic use)
• Animals
• Cell Line, Tumor
• Colonic Neoplasms (immunology, therapy)
• Cytokines (blood, immunology)
• Drug Carriers (chemistry)
• Hot Temperature
• Immunotherapy (methods)
• Male
• Mice, Inbred BALB C
• Microscopy, Atomic Force
• Nanotubes, Carbon (chemistry)
• Oligodeoxyribonucleotides (administration & dosage, pharmacology, therapeutic use)
• Spectroscopy, Near-Infrared
• Surface Properties