Echinochrome A protects mitochondrial function in cardiomyocytes against cardiotoxic drugs.

Abstract

Echinochrome A (Ech A) is a naphthoquinoid pigment from sea urchins that possesses antioxidant, antimicrobial, anti-inflammatory and chelating abilities. Although Ech A is the active substance in the ophthalmic and cardiac drug Histochrome®, its underlying cardioprotective mechanisms are not well understood. In this study, we investigated the protective role of Ech A against toxic agents that induce death of rat cardiac myoblast H9c2 cells and isolated rat cardiomyocytes. We found that the cardiotoxic agents tert-Butyl hydroperoxide (tBHP, organic reactive oxygen species (ROS) inducer), sodium nitroprusside (SNP; anti-hypertension drug), and doxorubicin (anti-cancer drug) caused mitochondrial dysfunction such as increased ROS level and decreased mitochondrial membrane potential. Co-treatment with Ech A, however, prevented this decrease in membrane potential and increase in ROS level. Co-treatment of Ech A also reduced the effects of these cardiotoxic agents on mitochondrial oxidative phosphorylation and adenosine triphosphate level. These findings indicate the therapeutic potential of Ech A for reducing cardiotoxic agent-induced damage.

• Authors: Seung Hun Jeong, Hyoung Kyu Kim, In-Sung Song, Seon Joong Lee, Kyung Soo Ko, Byoung Doo Rhee, Nari Kim, Natalia P Mishchenko, Sergey A Fedoryev, Valentin A Stonik, Jin Han
• Journal: Marine drugs (Mar Drugs) Vol. 12 Issue 5 Pg. 2922-36 (May 13 2014) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 24828295 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cardiotonic Agents
• Cardiotoxins
• Naphthoquinones
• Reactive Oxygen Species
• Adenosine Triphosphate
• Mitogen-Activated Protein Kinases
• echinochrome A

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Cardiotonic Agents (pharmacology)
• Cardiotoxins (antagonists & inhibitors, toxicity)
• Cell Death (drug effects)
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria, Heart (drug effects)
• Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism)
• Myocytes, Cardiac (drug effects)
• Naphthoquinones (pharmacology)
• Rats
• Reactive Oxygen Species (metabolism)
• Sea Urchins