Abstract |
Due to the high prevalence of iron and vitamin A deficiencies and to the controversy about the role of vitamin A and carotenoids in iron absorption, the objectives of this study were to evaluate the following: (1) the effect of a molar excess of vitamin A as well as the role of tannic acid on iron uptake by Caco-2 cells; (2) iron uptake and ferritin synthesis in presence of carotenoids without pro- vitamin A activity: lycopene, lutein, and zeaxantin; and (3) iron uptake and ferritin synthesis from ferrous fumarate and NaFe- EDTA. Cells were incubated 1 h at 37 °C in PBS pH 5.5, containing (59) Fe and different iron compounds. Vitamin A, ferrous fumarate, β- carotene, lycopene, lutein, zeaxantin, and tannic acid were added to evaluate uptake. Ferritin synthesis was measured 24 h after uptake experiments. Vitamin A had no effect on iron uptake by Caco-2 cells, and was significantly lower from NaFe- EDTA than from ferrous fumarate (15.2 ± 2.5 compared with 52.5 ± 8.3 pmol Fe/mg cell protein, respectively). Carotenoids increase uptake up to 50% from fumarate and up to 300% from NaFe- EDTA, since absorption from this compound is low when administered alone. We conclude the following: (1) There was no effect of vitamin A on iron uptake and ferritin synthesis by Caco-2cells. (2) Carotenoids significantly increased iron uptake from ferrous fumarate and NaFe- EDTA, and were capable of partially overcoming the inhibition produced by tannic acid. (3) Iron uptake by Caco-2 cell from NaFe- EDTA was significantly lower compared to other iron compounds, although carotenoids increased and tannic acid inhibited iron uptake comparably to ferrous fumarate.
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Authors | María N García-Casal, Irene Leets |
Journal | Journal of food science
(J Food Sci)
Vol. 79
Issue 4
Pg. H706-12
(Apr 2014)
ISSN: 1750-3841 [Electronic] United States |
PMID | 24665932
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 Institute of Food Technologists® |
Chemical References |
- Antioxidants
- Ferrous Compounds
- Iron Chelating Agents
- Iron Compounds
- Plant Extracts
- Tannins
- Vitamins
- Vitamin A
- Carotenoids
- Ferritins
- Edetic Acid
- Iron
- ferrous fumarate
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Topics |
- Antioxidants
(pharmacology)
- Caco-2 Cells
- Carotenoids
(pharmacology)
- Edetic Acid
(chemistry)
- Ferritins
(biosynthesis)
- Ferrous Compounds
(metabolism, pharmacology)
- Humans
- Intestinal Absorption
(drug effects)
- Iron
(metabolism)
- Iron Chelating Agents
(chemistry)
- Iron Compounds
(metabolism, pharmacology)
- Plant Extracts
(pharmacology)
- Tannins
(pharmacology)
- Vitamin A
(pharmacology)
- Vitamins
(pharmacology)
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