Abstract | BACKGROUND: METHODS: RESULTS: In patients without AKI and bacterial infection, at 1 week after SCT, the median blood neutrophil counts became zero and serum LCN2 levels were decreased by 76 ± 6 % (p < 0.01), but urinary LCN2 levels were not altered. During neutropenic conditions, bacterial infection caused only a modest rise of serum LCN2 but AKI produced a marked rise of serum and urinary LCN2 levels. Serum LCN2 concentrations in C/EBPε knockout mice were reduced by 66 ± 11 % compared to wild-type mice (p < 0.05). Blood LCN2 existed predominantly in high molecular weight forms (>100 kDa), while urinary LCN2 was mainly in low molecular weight forms. CONCLUSION: Our findings suggest that neutrophils are the major source of circulating LCN2 in normal and infected conditions, whereas blood and urinary LCN2 mainly derive from the kidney during AKI, and that the molecular forms and regulation of blood and urinary LCN2 are clearly distinct.
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Authors | Junya Kanda, Kiyoshi Mori, Hiroshi Kawabata, Takashige Kuwabara, Keita P Mori, Hirotaka Imamaki, Masato Kasahara, Hideki Yokoi, Chisaki Mizumoto, Nils H Thoennissen, H Phillip Koeffler, Jonathan Barasch, Akifumi Takaori-Kondo, Masashi Mukoyama, Kazuwa Nakao |
Journal | Clinical and experimental nephrology
(Clin Exp Nephrol)
Vol. 19
Issue 1
Pg. 99-106
(Feb 2015)
ISSN: 1437-7799 [Electronic] Japan |
PMID | 24599361
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acute-Phase Proteins
- Biomarkers
- CCAAT-Enhancer-Binding Proteins
- Cebpe protein, mouse
- Lipocalin-2
- Lipocalins
- Oncogene Proteins
- Lcn2 protein, mouse
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Topics |
- Acute Kidney Injury
(blood)
- Acute-Phase Proteins
(urine)
- Animals
- Bacterial Infections
(blood, urine)
- Biomarkers
(blood, urine)
- CCAAT-Enhancer-Binding Proteins
(genetics, physiology)
- Hematologic Neoplasms
(complications, therapy)
- Hematopoietic Stem Cell Transplantation
- Humans
- Kidney
(metabolism)
- Lipocalin-2
- Lipocalins
(blood, urine)
- Mice
- Mice, Knockout
- Molecular Weight
- Neutrophils
(metabolism)
- Oncogene Proteins
(blood, urine)
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