Guanine nucleotide-binding protein subunit beta-2-like 1, a new Annexin A7 interacting protein.

Abstract

We report for the first time that Guanine nucleotide-binding protein subunit beta-2-like 1 (RACK1) formed a complex with Annexin A7. Hca-F and Hca-P are a pair of syngeneic mouse hepatocarcinoma cell lines established and maintained in our laboratory. Our previous study showed that both Annexin A7 and RACK1 were expressed higher in Hca-F (lymph node metastasis >70%) than Hca-P (lymph node metastasis <30%). Suppression of Annexin A7 expression in Hca-F cells induced decreased migration and invasion ability. In this study, knockdown of RACK1 by RNA interference (RNAi) had the same impact on metastasis potential of Hca-F cells as Annexin A7 down-regulation. Furthermore, by co-immunoprecipitation and double immunofluorescence confocal imaging, we found that RACK1 was in complex with Annexin A7 in control cells, but not in the RACK1-down-regulated cells, indicating the abolishment of RACK1-Annexin A7 interaction in Hca-F cells by RACK1 RNAi. Taken together, these results suggest that RACK1-Annexin A7 interaction may be one of the means by which RACK1 and Annexin A7 influence the metastasis potential of mouse hepatocarcinoma cells in vitro.

• Authors: Yue Du, Jinyi Meng, Yuhong Huang, Jun Wu, Bo Wang, Mohammed M Ibrahim, Jianwu Tang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 445 Issue 1 Pg. 58-63 (Feb 28 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 24491534 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Annexin A7
• Neuropeptides
• RACK1 protein, mouse
• Receptors for Activated C Kinase

Topics

• Animals
• Annexin A7 (genetics, metabolism)
• Blotting, Western
• Carcinoma, Hepatocellular (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation
• Gene Expression Regulation, Neoplastic
• Liver Neoplasms (genetics, metabolism, pathology)
• Lymphatic Metastasis
• Mice
• Microscopy, Confocal
• Neuropeptides (genetics, metabolism)
• Protein Binding
• RNA Interference
• Receptors for Activated C Kinase
• Reverse Transcriptase Polymerase Chain Reaction