Increase of Myoglobin in Rat Gastrocnemius Muscles with Immobilization-induced Atrophy.

Abstract

[Purpose] Atrophy is a common phenomenon caused by prolonged muscle disuse associated with bed-rest, aging, and immobilization. However, changes in the expression of atrophy-related myoglobin are still poorly understood. In the present study, we examined whether or not myoglobin expression is altered in the gastrocnemius muscles of rats after seven days of cast immobilization. [Methods] We conducted a protein expression and high-resolution differential proteomic analysis using, two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry, and western blotting. [Results] The density and expression of myoglobin increased significantly more in atrophic gastrocnemius muscle strips than they did in the control group. [Conclusion] The results suggest that cast immobilization-induced atrophy may be related to changes in the expression of myoglobin in rat gastrocnemius muscles.

• Authors: Jeong-Uk Lee, Ju-Hyun Kim, Mee-Young Kim, Lim-Kyu Lee, Seung-Min Yang, Hye-Joo Jeon, Won-Deok Lee, Ji-Woong Noh, Tae-Hyun Lee, Taek-Yong Kwak, Bokyung Kim, Junghwan Kim
• Journal: Journal of physical therapy science (J Phys Ther Sci) Vol. 25 Issue 12 Pg. 1617-20 (Dec 2013) ISSN: 0915-5287 [Print] Japan
• PMID: 24409033 (Publication Type: Journal Article)