Effect of nimesulide on the growth of human laryngeal squamous cell carcinoma.

Abstract

PURPOSE:

To investigate the effect of nimesulide on the growth of human laryngeal squamous cell carcinoma.

MATERIALS AND METHODS:

The effect of NIM on Hep-2 cell proliferation was measured by the MTT assay. Flow cytometry was used to evaluate the cell cycle and apoptosis in Hep-2 cells. A Western blot analysis was used to detect changes in the protein expression levels of COX-2, Survivin and proliferating cell nuclear antigen (PCNA) in Hep-2 cells. A Hep-2 tumor xenograft model was established in nude mice to observe tumor growth. The changes in the xenograft tumors were observed after hematoxylin/eosin staining. The expression levels of COX-2, Survivin and PCNA proteins and mRNA were measured by immunohistochemical analysis and RT-PCR, respectively.

RESULTS:

NIM had time- and dose-dependent inhibitory effect on the proliferation of Hep-2 cells. NIM could prevent the progression of the cell cycle. After NIM treatment, COX-2, Survivin and PCNA protein levels were reduced in the Hep-2 cells. The volume and weight of the xenograft tumors in the NIM treatment group were significantly reduced. The NIM treatment group also exhibited significantly reduced expression levels of COX-2, Survivin and PCNA at both the protein and mRNA levels.

CONCLUSIONS:

Our results suggested that NIM has significant inhibitory effects on the growth of Hep-2 cells and xenograft tumors in nude mice. Selective COX-2 inhibitors could potentially become part of a comprehensive treatment for laryngeal squamous cell carcinoma. Additional research and development will provide new and broader prospects for the prevention and treatment of laryngeal squamous cell carcinoma.

• Authors: Zhuoping Liang, Jinbo Liu, Leiji Li, Haiyang Wang, Chong Zhao, Liang Jiang, Gang Qin
• Journal: American journal of otolaryngology (Am J Otolaryngol) 2014 Mar-Apr Vol. 35 Issue 2 Pg. 120-9 ISSN: 1532-818X [Electronic] United States
• PMID: 24321753 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014.

Chemical References

• Birc5 protein, mouse
• Cyclooxygenase Inhibitors
• Inhibitor of Apoptosis Proteins
• Platelet Aggregation Inhibitors
• Proliferating Cell Nuclear Antigen
• RNA, Messenger
• Repressor Proteins
• Sulfonamides
• Survivin
• Ptgs2 protein, mouse
• Cyclooxygenase 2
• nimesulide

Topics

• Animals
• Apoptosis (drug effects)
• Blotting, Western
• Carcinoma, Squamous Cell (drug therapy, genetics, pathology)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation
• Cyclooxygenase 2 (biosynthesis, genetics)
• Cyclooxygenase Inhibitors (pharmacology)
• Flow Cytometry
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Immunohistochemistry
• Inhibitor of Apoptosis Proteins (biosynthesis, genetics)
• Laryngeal Neoplasms (drug therapy, genetics, pathology)
• Mice
• Mice, Inbred BALB C
• Neoplasms, Experimental (drug therapy, genetics, pathology)
• Platelet Aggregation Inhibitors
• Proliferating Cell Nuclear Antigen (biosynthesis, genetics)
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction
• Repressor Proteins (biosynthesis, genetics)
• Sulfonamides (pharmacology)
• Survivin