Abstract | PURPOSE: Wear debris particle-induced osteolysis and subsequent aseptic loosening is one of the major causes of failure of total joint replacement. The purpose of this study was to investigate the effect of titanium implant material and inflammatory cytokines on human synovial cells and the development to osteolysis and aseptic loosening. METHODS: This study investigated the effect of titanium implant material on the ECM-degraded MMP-2 in human synovial cells and analyzed the contribution of synovial cells in osteolysis and aseptic loosening. RESULTS: When human synovial cells are exposed to titanium materials, MMP-2 activity is induced by 1.72 ± 0.14-fold with Ti disc and 3.95 ± 0.10-fold with Ti particles, compared with that of the controls, respectively. Inflammatory cytokines TNFα and IL-1β are also shown to induce MMP-2 activity by 3.65 ± 0.28-fold and 6.76 ± 0.28-fold, respectively. A combination of Ti particles and cytokines induces MMP-2 activities to a higher level (10.54 ± 0.45-fold). Inhibitors of various signal pathways involved in MMP-2 reverse Ti particle-induced MMP-2 activities. CONCLUSIONS:
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Authors | Chunfeng Fu, Jing Xie, Ning Hu, Xi Liang, Rongfu Chen, Chunli Wang, Cheng Chen, Chunming Xu, Wei Huang, K-L Paul Sung |
Journal | International orthopaedics
(Int Orthop)
Vol. 38
Issue 5
Pg. 1091-8
(May 2014)
ISSN: 1432-5195 [Electronic] Germany |
PMID | 24271334
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Titanium
- Matrix Metalloproteinase 2
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Topics |
- Adult
- Cells, Cultured
- Humans
- Matrix Metalloproteinase 2
(biosynthesis, physiology)
- Middle Aged
- Synovial Membrane
(cytology)
- Titanium
(pharmacology)
- Up-Regulation
(drug effects)
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