Research of the methylation status of miR-124a gene promoter among rheumatoid arthritis patients.

Abstract

OBJECTIVE:

To analyze the methylation status of miR-124a loci in synovial tissues of rheumatoid arthritis (RA) patients using methylation-specific polymerase chain reaction (MSP).

MATERIALS AND METHODS:

DNA obtained from the frozen tissue of 7 RA samples, 6 osteoarthritis (OA) samples, and 3 healthy controls were undergoing bisulfite conversion and then analyzed for miR-124a promoter methylation using MSP assay.

RESULTS:

miR-124-a1 and miR-124-a2 promoter methylation were both seen in 71.4% of RA samples compared to 16.7% of OA samples. miR-124-a3 promoter methylation was seen in 57.1% of RA samples and 0% of OA samples. All the three loci were unmethylated in 3 healthy controls.

CONCLUSION:

The methylation status of miR-124a seen in this study concurs with that reported in tumor cells, indicating epigenetic dysregulation constituents, a mechanism in the development of rheumatoid arthritis.

• Authors: Qiao Zhou, Li Long, Guixiu Shi, Jing Zhang, Tong Wu, Bin Zhou
• Journal: Clinical & developmental immunology (Clin Dev Immunol) Vol. 2013 Pg. 524204 ( 2013) ISSN: 1740-2530 [Electronic] Egypt
• PMID: 24223605 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN124 microRNA, human
• MicroRNAs

Topics

• Adult
• Aged
• Arthritis, Rheumatoid (genetics)
• DNA Methylation
• Female
• Humans
• Male
• MicroRNAs (genetics)
• Middle Aged
• Osteoarthritis (genetics)
• Promoter Regions, Genetic