Abstract | BACKGROUND: METHODS: RESULTS: A 48 hours exposure to indoxyl sulfate significantly increased [Ca(2+)]i (≥ 300 μM), significantly decreased forward scatter (≥ 300 μM) and significantly increased annexin-V-binding (≥ 50 μM). Indoxyl sulfate (150 μM) induced annexin-V-binding was virtually abolished in the nominal absence of extracellular Ca(2+). Indoxyl sulfate (150 μM) further enhanced ceramide abundance. CONCLUSION:
Indoxyl sulfate stimulates suicidal erythrocyte death or eryptosis, an effect in large part due to stimulation of extracellular Ca(2+)entry with subsequent stimulation of cell shrinkage and cell membrane scrambling.
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Authors | Mohamed Siyabeldin E Ahmed, Majed Abed, Jakob Voelkl, Florian Lang |
Journal | BMC nephrology
(BMC Nephrol)
Vol. 14
Pg. 244
(Nov 04 2013)
ISSN: 1471-2369 [Electronic] England |
PMID | 24188099
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hemolytic Agents
- Toxins, Biological
- uremia middle molecule toxins
- Indican
- Calcium
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Topics |
- Apoptosis
(drug effects, physiology)
- Calcium
(metabolism)
- Cell Size
(drug effects)
- Cells, Cultured
- Erythrocyte Membrane
(drug effects, physiology)
- Erythrocytes
(drug effects, physiology)
- Genes, Transgenic, Suicide
- Hemolysis
(drug effects, physiology)
- Hemolytic Agents
(pharmacology)
- Humans
- Indican
(pharmacology)
- Toxins, Biological
(pharmacology)
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