Perspective on how to approach molecular diagnostics in acute myeloid leukemia and myelodysplastic syndromes in the era of next-generation sequencing.

Abstract

Molecular mutation information became essential for biological subclassification, risk stratification and therapeutic decisions in patients with acute myeloid leukemia (AML). In myelodysplastic syndromes (MDS), a broad spectrum of molecular biomarkers such as the spliceosome mutations has been identified in recent years. The currently established combination of various polymerase chain reaction (PCR) methods with capillary Sanger sequencing for mutation analysis in AML is time-consuming and labor-intensive. The constantly increasing spectrum of molecular mutations is a tremendous challenge for hematological laboratories. The introduction of high-throughput sequencing technology, which allows the massive parallel analysis of hundreds of thousands of alleles in the shortest time, provides new options for molecular mutation analyses and for follow-up diagnostics in myeloid neoplasms. In contrast to whole-genome or exome analyses, amplicon deep-sequencing focuses on distinct genomic loci and their mutation patterns and enables a comprehensive biomarker analysis in a multitude of patients per analysis. This review summarizes thus far established common molecular diagnostic strategies and intends to outline the perspective of distinct novel amplicon deep-sequencing panels for patients with AML and MDS. It is foreseeable that clearly defined algorithms for molecular investigations will revolutionize diagnosis in patients with AML and MDS in the near future.

• Authors: Alexander Kohlmann, Ulrike Bacher, Susanne Schnittger, Torsten Haferlach
• Journal: Leukemia & lymphoma (Leuk Lymphoma) Vol. 55 Issue 8 Pg. 1725-34 (Aug 2014) ISSN: 1029-2403 [Electronic] United States
• PMID: 24144312 (Publication Type: Journal Article, Review)

Chemical References

• Biomarkers, Tumor
• Genetic Markers

Topics

• Algorithms
• Biomarkers, Tumor
• DNA Mutational Analysis
• Diagnostic Tests, Routine
• Genetic Markers
• Genetic Testing (methods, standards)
• Genomics (methods, standards)
• High-Throughput Nucleotide Sequencing
• Humans
• Leukemia, Myeloid, Acute (diagnosis, genetics)
• Mutation
• Myelodysplastic Syndromes (diagnosis, genetics)
• Sensitivity and Specificity