A functional variant of pre-miRNA-196a2 confers risk for Behcet's disease but not for Vogt-Koyanagi-Harada syndrome or AAU in ankylosing spondylitis.

Abstract

This study aimed to investigate the predisposition of common pre-miRNA SNPs with Behcet's disease (BD), Vogt-Koyanagi-Harada (VKH) syndrome and acute anterior uveitis (AAU) associated with ankylosing spondylitis (AS). A two-stage association study was carried out in 859 BD, 400 VKH syndrome, 209 AAU(+)AS(+) patients and 1,685 controls all belonging to a Chinese Han population. Genotyping, the expression of miR-196a and Bach1 (the target gene of miR-196a), cell proliferation, cytokine production were examined by PCR-RFLP, real-time PCR, CCK8 and ELISA. In the first stage study, the results showed significantly increased frequencies of the miR-196a2/rs11614913 TT genotype and T allele in BD patients (adjusted P(c) = 0.024, OR = 1.63; adjusted P(c) = 5.4 × 10(-3), OR = 1.45, respectively). However, no significant association of the tested SNPs with VKH and AAU(+)AS(+) patients was observed. The second stage and combined studies confirmed the association of rs11614913 with BD (TT genotype: adjusted P(c) = 6×10(-5), OR = 1.53; T allele: adjusted P(c) = 8×10(-6), OR = 1.35; CC genotype: adjusted P(c) = 0.024, OR = 0.68). A stratified analysis showed an association of the rs11614913 TT genotype and T allele with the arthritis subgroup of BD (P(c) = 5.3 × 10(-3), OR = 1.89; P(c) = 0.015, OR = 1.56, respectively). Functional experiments showed a decreased miR-196a expression, an increased Bach1 expression and an increased production of IL-1β and MCP-1 in TT cases compared to CC cases (P = 0.023, P = 0.0073, P = 0.012, P = 0.002, respectively). This study shows that a functional variant of miR-196a2 confers risk for BD but not for VKH syndrome or AAU(+)AS(+) by modulating the miR-196a gene expression and by regulating pro-inflammatory IL-1β and MCP-1 production.

• Authors: Jian Qi, Shengping Hou, Qi Zhang, Dan Liao, Lin Wei, Jing Fang, Yan Zhou, Aize Kijlstra, Peizeng Yang
• Journal: Human genetics (Hum Genet) Vol. 132 Issue 12 Pg. 1395-404 (Dec 2013) ISSN: 1432-1203 [Electronic] Germany
• PMID: 23928854 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MIRN196 microRNA, human
• MicroRNAs
• RNA Precursors

Topics

• Acute Disease
• Behcet Syndrome (epidemiology, genetics)
• Case-Control Studies
• Cells, Cultured
• Female
• Gene Frequency
• Genetic Predisposition to Disease
• Humans
• Male
• MicroRNAs (genetics)
• Phenotype
• Polymorphism, Single Nucleotide (physiology)
• RNA Precursors (genetics)
• Risk Factors
• Spondylitis, Ankylosing (complications, epidemiology, genetics)
• Uveitis, Anterior (complications, epidemiology, genetics)
• Uveomeningoencephalitic Syndrome (epidemiology, genetics)