Profiling celiac disease antibody repertoire.

Abstract

The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by protein microarray. The specificity for 6 of these antigens was confirmed by ELISA. Furthermore we showed that this antibody response was not abolished on a gluten free diet and was not shared with other autoimmune diseases. These antigens appear to be CD specific and independent of gluten induction. The utility of this panel extends beyond its diagnostic value and it may drive the attention to new targets for unbiased screens in autoimmunity research.

• Authors: Sara D'Angelo, Flavio Mignone, Cecilia Deantonio, Roberto Di Niro, Roberta Bordoni, Roberto Marzari, Gianluca De Bellis, Tarcisio Not, Fortunato Ferrara, Andrew Bradbury, Claudio Santoro, Daniele Sblattero
• Journal: Clinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 148 Issue 1 Pg. 99-109 (Jul 2013) ISSN: 1521-7035 [Electronic] United States
• PMID: 23685219 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Autoantibodies
• Autoantigens

Topics

• Adolescent
• Adult
• Autoantibodies (blood, immunology)
• Autoantigens (genetics, immunology)
• Celiac Disease (blood, diagnosis, genetics, immunology)
• Cell Surface Display Techniques
• Child
• Child, Preschool
• Female
• Humans
• Infant
• Male
• Middle Aged
• Oligonucleotide Array Sequence Analysis
• Protein Array Analysis
• ROC Curve
• Young Adult