Abstract | BACKGROUND: METHODS: For in vitro studies, blood and lymphatic endothelial cells were treated with tafluprost; cell proliferation was assessed after 48 h. For long-term in vivo studies under healthy conditions, naïve corneas of BALB/c mice were treated with tafluprost eye drops for 4 weeks. For short-term in vivo studies under inflammatory conditions, corneal inflammation was induced by suture placement; mice then received tafluprost eye drops for 1 week. Afterwards, corneas were stained with CD31 as panendothelial and LYVE-1 as lymphendothelial (and macrophage) marker. RESULTS: In vitro, tafluprost did not alter blood or lymphatic endothelial cell proliferation. In vivo, there was no change in limbal blood or lymphatic vessel anatomy after long-term treatment with tafluprost. Short-term treatment with tafluprost under inflammatory conditions did not influence the recruitment of LYVE-1 positive macrophages into the cornea. Moreover, treatment of inflamed corneas with tafluprost did not significantly influence corneal hem- and lymphangiogenesis. CONCLUSIONS:
Tafluprost does not affect blood and lymphatic vessel growth, neither under resting nor under inflammatory conditions. These findings suggest a safe vascular profile of tafluprost eye drops at the inflammatory neovascularized cornea.
|
Authors | Deniz Hos, Konrad R Koch, Felix Bock, Rafael S Grajewski, Thomas S Dietlein, Claus Cursiefen, Ludwig M Heindl |
Journal | Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
(Graefes Arch Clin Exp Ophthalmol)
Vol. 251
Issue 8
Pg. 1919-27
(Aug 2013)
ISSN: 1435-702X [Electronic] Germany |
PMID | 23624591
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antihypertensive Agents
- Biomarkers
- Glycoproteins
- Membrane Transport Proteins
- Ophthalmic Solutions
- Platelet Endothelial Cell Adhesion Molecule-1
- Prostaglandins F
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factor C
- Xlkd1 protein, mouse
- vascular endothelial growth factor A, mouse
- vascular endothelial growth factor C, mouse
- tafluprost
|
Topics |
- Administration, Topical
- Animals
- Antihypertensive Agents
(pharmacology)
- Biomarkers
(metabolism)
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Cornea
(blood supply)
- Corneal Neovascularization
(metabolism, physiopathology)
- Disease Models, Animal
- Endothelium, Vascular
(drug effects)
- Enzyme-Linked Immunosorbent Assay
- Female
- Glycoproteins
(metabolism)
- Keratitis
(metabolism, physiopathology)
- Lymphangiogenesis
(drug effects)
- Lymphatic Vessels
(drug effects, pathology)
- Membrane Transport Proteins
- Mice
- Mice, Inbred BALB C
- Ophthalmic Solutions
- Platelet Endothelial Cell Adhesion Molecule-1
(metabolism)
- Prostaglandins F
(pharmacology)
- Vascular Endothelial Growth Factor A
(pharmacology)
- Vascular Endothelial Growth Factor C
(pharmacology)
|