Abstract |
Using 15 rats with collagen-induced arthritis (30 joints) and 7 control rats (14 joints), we correlated the intensity of near-infrared fluorescence (NIRF) of the nonspecific dye tetrasulfocyanine ( TSC) with magnetic resonance imaging (MRI), histopathology, and clinical score. Fluorescence images were obtained in reflection geometry using a NIRF camera system. Normalized fluorescence intensity (INF) was determined after intravenous dye administration on different time points up to 120 min. Contrast-enhanced MRI using gadodiamide was performed after NIRF imaging. Analyses were performed in a blinded fashion. Histopathological and clinical scores were determined for each ankle joint. INF of moderate and high-grade arthritic joints were significantly higher (p<0.005) than the values of control and low-grade arthritic joints between 5 and 30 min after TSC-injection. This result correlated well with post-contrast MRI signal intensities at about 5 min after gadodiamide administration. Furthermore, INF and signal increase on contrast-enhanced MRI showed high correlation with clinical and histopathological scores. Sensitivities and specificities for detection of moderate and high-grade arthritic joints were slightly lower for NIRF imaging (89%/81%) than for MRI (100%/91%). NIRF imaging using TSC, which is characterized by slower plasma clearance compared to indocyanine green (ICG), has the potential to improve monitoring of inflamed joints.
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Authors | Ines Gemeinhardt, Dorothee Puls, Ole Gemeinhardt, Matthias Taupitz, Susanne Wagner, Beatrix Schnorr, Kai Licha, Michael Schirner, Bernd Ebert, Diethard Petzelt, Rainer Macdonald, Jörg Schnorr |
Journal | Journal of biomedical optics
(J Biomed Opt)
Vol. 17
Issue 10
Pg. 106008
(Oct 2012)
ISSN: 1560-2281 [Electronic] United States |
PMID | 23224007
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carbocyanines
- Contrast Media
- Fluorescent Dyes
- Gadolinium
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Topics |
- Animals
- Arthritis, Experimental
(metabolism, pathology)
- Carbocyanines
(chemistry, pharmacokinetics)
- Contrast Media
(chemistry, pharmacokinetics)
- Female
- Fluorescent Dyes
(chemistry, pharmacokinetics)
- Gadolinium
(chemistry, pharmacokinetics)
- Histocytochemistry
- Joints
(anatomy & histology, chemistry, metabolism, pathology)
- Magnetic Resonance Imaging
(methods)
- Optical Imaging
- Rats
- Spectroscopy, Near-Infrared
(methods)
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