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Near-infrared fluorescence imaging of experimentally collagen-induced arthritis in rats using the nonspecific dye tetrasulfocyanine in comparison with gadolinium-based contrast-enhanced magnetic resonance imaging, histology, and clinical score.

Abstract
Using 15 rats with collagen-induced arthritis (30 joints) and 7 control rats (14 joints), we correlated the intensity of near-infrared fluorescence (NIRF) of the nonspecific dye tetrasulfocyanine (TSC) with magnetic resonance imaging (MRI), histopathology, and clinical score. Fluorescence images were obtained in reflection geometry using a NIRF camera system. Normalized fluorescence intensity (INF) was determined after intravenous dye administration on different time points up to 120 min. Contrast-enhanced MRI using gadodiamide was performed after NIRF imaging. Analyses were performed in a blinded fashion. Histopathological and clinical scores were determined for each ankle joint. INF of moderate and high-grade arthritic joints were significantly higher (p<0.005) than the values of control and low-grade arthritic joints between 5 and 30 min after TSC-injection. This result correlated well with post-contrast MRI signal intensities at about 5 min after gadodiamide administration. Furthermore, INF and signal increase on contrast-enhanced MRI showed high correlation with clinical and histopathological scores. Sensitivities and specificities for detection of moderate and high-grade arthritic joints were slightly lower for NIRF imaging (89%/81%) than for MRI (100%/91%). NIRF imaging using TSC, which is characterized by slower plasma clearance compared to indocyanine green (ICG), has the potential to improve monitoring of inflamed joints.
AuthorsInes Gemeinhardt, Dorothee Puls, Ole Gemeinhardt, Matthias Taupitz, Susanne Wagner, Beatrix Schnorr, Kai Licha, Michael Schirner, Bernd Ebert, Diethard Petzelt, Rainer Macdonald, Jörg Schnorr
JournalJournal of biomedical optics (J Biomed Opt) Vol. 17 Issue 10 Pg. 106008 (Oct 2012) ISSN: 1560-2281 [Electronic] United States
PMID23224007 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Carbocyanines
  • Contrast Media
  • Fluorescent Dyes
  • Gadolinium
Topics
  • Animals
  • Arthritis, Experimental (metabolism, pathology)
  • Carbocyanines (chemistry, pharmacokinetics)
  • Contrast Media (chemistry, pharmacokinetics)
  • Female
  • Fluorescent Dyes (chemistry, pharmacokinetics)
  • Gadolinium (chemistry, pharmacokinetics)
  • Histocytochemistry
  • Joints (anatomy & histology, chemistry, metabolism, pathology)
  • Magnetic Resonance Imaging (methods)
  • Optical Imaging
  • Rats
  • Spectroscopy, Near-Infrared (methods)

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