[Effect of flavin adenine dinucleotide on ultraviolet B induced damage in cultured human corneal epithelial cells].

Abstract

This study evaluated the effects of flavin adenine dinucleotide (FAD) on ultraviolet B (UV-B)-induced damage in cultured human corneal epithelial (HCE-T) cells. The cultured HCE-T cells were treated with 0.003125-0.05% FAD before exposure to 80 mJ/cm2 UV-B. Cell viability was measured 24 h after UV-B irradiation using the MTS assay. Reactive oxygen species (ROS) were detected 30 min after UV-B irradiation using 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate acetyl ester. Apoptosis was evaluated 4 h after UV-B irradiation in the caspase-3/7 activity assay. UV-B irradiation reduced cell viability and stimulated ROS production and caspase-3/7 activity in HCE-T cells. Pretreatment of UV-B irradiated HCE-T cells with FAD significantly attenuated cell viability reduction and inhibited the stimulation of both ROS production and caspase-3/7 activity due to UV-B exposure compared with those with vehicle (0% FAD). These results clarified that FAD inhibits ROS-mediated apoptosis by UV-B irradiation in HCE-T cells and suggest that FAD may be effective as a radical scavenger in UV-B-induced corneal damage.

• Authors: Asuka Sakamoto, Masatsugu Nakamura
• Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (Yakugaku Zasshi) Vol. 132 Issue 8 Pg. 933-7 ( 2012) ISSN: 1347-5231 [Electronic] Japan
• PMID: 22864352 (Publication Type: Journal Article)

Chemical References

• Flavin-Adenine Dinucleotide
• CASP3 protein, human
• CASP7 protein, human
• Caspase 3
• Caspase 7

Topics

• Caspase 3 (metabolism)
• Caspase 7 (metabolism)
• Cell Survival (drug effects, radiation effects)
• Cells, Cultured
• Cornea (drug effects, enzymology, radiation effects)
• Epithelial Cells (drug effects, enzymology, radiation effects)
• Flavin-Adenine Dinucleotide (pharmacology)
• Humans
• Ultraviolet Rays