Abstract | OBJECTIVES: METHODS: We have synthesized a new HDAC inhibitor, IN-2001, and cell proliferation inhibition assay with this chemical in estrogen receptor-positive human breast cancer MCF-7 cells. Cell cycle analysis on MCF-7 cells treated with IN-2001 was carried out by flow cytometry and gene expression was measured by RT-PCR. RESULTS: In MCF-7 cells IN-2001 showed remarkable anti-proliferative effects in a dose- and time-dependent manner. In MCF-7 cells, IN-2001 showed a more potent growth inhibitory effect than that of suberoylanilide hydroxamic acid. These growth inhibitory effects were related to the cell cycle arrest and induction of apoptosis. IN-2001 showed accumulation of cells at G(2)/M phase and of the sub-G(1) population in a time-dependent manner, representing apoptotic cells. IN-2001-mediated cell cycle arrest was associated with HDAC inhibitor-mediated induction of CDK inhibitor expression. In MCF-7 cells, IN-2001 significantly increased p21(WAF1) expression. CONCLUSIONS:
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Authors | Kyung Nan Min, Ki Eun Joung, Dae-Kee Kim, Yhun Yhong Sheen |
Journal | Environmental health and toxicology
(Environ Health Toxicol)
Vol. 27
Pg. e2012010
( 2012)
ISSN: 2233-6567 [Electronic] Korea (South) |
PMID | 22639737
(Publication Type: Journal Article)
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