[Effects of polychlorinated biphenyl on the expression of c-fos and c-jun in mesenchymal cells of rats].

Abstract

OBJECTIVE:

To study the effects of polychlorinated biphenyl (PCB126) on the expression of c-fos and c-jun in mesenchymal cells (MSCs) of rats.

METHODS:

MSCs were separated by percoll cell separating medium (density 1.073 g/cm3). After being cultured and transferred, the cells were divided into a control group, a low-dose group (10(-7) mol/L PCB126) and a high-dose group (10(-6) mol/L PCB126). The cellular growth rate and the expression of c-fos and c-jun protein were analyzed by MTT, immunofluorescence chemical assay, RT-PCR and Western blot.

RESULTS:

The cellular growth rate in the low-and high-dose group were 13.8% and 19.1% at 12 h, 31.5% and 36.1% at 24 h, 42.5% and 43.6% at 48 h, respectively (P < 0.01). The positive rates of c-fos expression in the low-and high-dose groups were 54.6% and 51.3% at 12 h, and 83.2% and 73.0% at 24 h. The expression of c-fos mRNA detected by RT-PCR was upregulated at 30 min and 1h in PCB126 groups. The expression of c-jun mRNA were much higher in PCB126 groups than that in the control group. The expression of c-fos and c-jun protein was upregulated in the low-and high-dose groups at 12 h and 24 h.

CONCLUSION:

PCB126 could promote the proliferation of MSCs and upregulate the expression of cancer associated gene c-fos and c-jun. The effect of PCB126 on the function of MSCs might be associated with the abnormal expression of c-fos and c-jun gene.

• Authors: Hongbin Zhao, Jianfeng Wang, Xu Wang, Juzi Dong, Huanfa Zhou, Yinshu Yang
• Journal: Wei sheng yan jiu = Journal of hygiene research (Wei Sheng Yan Jiu) Vol. 41 Issue 2 Pg. 180-4, 190 (Mar 2012) ISSN: 1000-8020 [Print] China
• PMID: 22611921 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Environmental Pollutants
• Proto-Oncogene Proteins c-fos
• Proto-Oncogene Proteins c-jun
• RNA, Messenger
• Polychlorinated Biphenyls
• 3,4,5,3',4'-pentachlorobiphenyl

Topics

• Animals
• Bone Marrow Cells (cytology)
• Environmental Pollutants (toxicity)
• Female
• Male
• Mesenchymal Stem Cells (cytology, metabolism)
• Polychlorinated Biphenyls (toxicity)
• Proto-Oncogene Proteins c-fos (genetics, metabolism)
• Proto-Oncogene Proteins c-jun (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Wistar
• Up-Regulation (drug effects)