Abstract |
Polyphenols from extra virgin olive oil (EVOO), a main component of the Mediterranean diet, have demonstrated repeatedly anti- tumor activity in several in vitro and in vivo studies. However, little is known about the efficiency of the absorption process and metabolic conversion of these compounds at cellular level. In this study, a nano liquid chromatography-electrospray ionization-time of flight mass spectrometry (nanoLC-ESI-TOF MS) method was developed to study the cellular uptake and metabolism of olive oil phenols in JIMT-1 human breast cancer cells. After incubation for different time periods with EVOO-derived phenolic extracts, culture media, cytosolic fraction and solid particles fraction were separated and analyzed. Most of the free phenols, mainly hydroxytyrosol, its secoiridoid derivatives, and the flavonoid luteolin, disappeared in the culture media in different ways and at different times. Besides, several metabolites were detected in the culture media, fact that may indicate absorption and intracellular metabolism followed by rapid cellular export. Low intracellular accumulation was observed with only traces of some compounds detected in the cytosolic and solid particles fractions. Methylated conjugates were the major metabolites detected, suggesting a catalytic action of catechol-O-methyl transferase (COMT) in cancer cells.
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Authors | Rocío García-Villalba, Alegría Carrasco-Pancorbo, Cristina Oliveras-Ferraros, Javier A Menéndez, Antonio Segura-Carretero, Alberto Fernández-Gutiérrez |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 898
Pg. 69-77
(Jun 01 2012)
ISSN: 1873-376X [Electronic] Netherlands |
PMID | 22608806
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier B.V. All rights reserved. |
Chemical References |
- Flavonoids
- Iridoids
- Lignans
- Olive Oil
- Plant Extracts
- Plant Oils
- Polyphenols
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Topics |
- Breast Neoplasms
(metabolism, pathology)
- Cell Line, Tumor
- Chromatography, Liquid
(methods)
- Female
- Flavonoids
(chemistry, metabolism, pharmacokinetics)
- Humans
- Iridoids
(chemistry, metabolism, pharmacokinetics)
- Lignans
(chemistry, metabolism, pharmacokinetics)
- Middle Aged
- Nanotechnology
- Olive Oil
- Plant Extracts
(metabolism, pharmacokinetics)
- Plant Oils
(chemistry)
- Polyphenols
(chemistry, metabolism, pharmacokinetics)
- Spectrometry, Mass, Electrospray Ionization
(methods)
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