Recruitment of circulating dendritic cell precursors into the infarcted myocardium and pro-inflammatory response in acute myocardial infarction.

Abstract

DC (dendritic cells) play an important role in the immune system. They invade peripheral tissues to detect harmful antigens, inducing a local immune response. Studies suggest that DCPs (dendritic cell precursors) might be reduced in AMI (acute myocardial infarction); however, the reason for their reduction is unknown yet. In the present study, circulating mDCPs (myeloid DCPs), pDCPs (plasmacytoid DCPs), tDCPs (total DCPs) and serum levels of TNFα (tumour necrosis factor α), IL (interleukin)-2, -4, -5, -6, -10 and -12 were analysed by flow cytometry in blood of patients with NSTEMI [non-STEMI (ST-segment elevation myocardial infarction)] (n=44) and STEMI (n=34) compared with controls with excluded CAD (coronary artery disease) (n=45). Post-mortem myocardial specimens of patients with AMI (n=12) and healthy myocardium of accident victims (n=10) were immunostained for mDCs (myeloid dendritic cells) T-cells and macrophages. Compared with controls, in patients with AMI a significant decrease in circulating mDCPs, pDCPs and tDCPs was observed (each P<0.0001). The extent of the decrease was higher in STEMI than NSTEMI patients. Serum levels were significantly higher in patients with AMI compared with controls for IL-6, -10, -12 and TNFα (each P<0.03). Immunostaining revealed significantly higher number of DCs, T-cells and macrophages (each P<0.002) in infarcted than control myocardium. We show that circulating DCPs are significantly reduced in AMI, with a pronounced reduction in STEMI patients. This was accompanied by a significant increase of inflammatory serum cytokines in patients with AMI. Immunohistochemical analysis unravelled that the reduction of circulating DCPs might be due to recruitment into the infarcted myocardium.

• Authors: Daniel Kretzschmar, Stefan Betge, Alexander Windisch, Rudin Pistulli, Ilonka Rohm, Michael Fritzenwanger, Christian Jung, Katja Schubert, Bernhard Theis, Iver Petersen, Stefanie Drobnik, Gita Mall, Hans-Reiner Figulla, Atilla Yilmaz
• Journal: Clinical science (London, England : 1979) (Clin Sci (Lond)) Vol. 123 Issue 6 Pg. 387-98 (Sep 2012) ISSN: 1470-8736 [Electronic] England
• PMID: 22494099 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Tumor Necrosis Factor-alpha

Topics

• Aged
• Cytokines (blood)
• Dendritic Cells (pathology, physiology)
• Female
• Flow Cytometry
• Humans
• Macrophages (pathology)
• Male
• Middle Aged
• Myocardial Infarction (blood, immunology)
• T-Lymphocytes (pathology)
• Tumor Necrosis Factor-alpha (blood)