[Caspase-independent apoptosis induced by arsenic trioxide in human multiple myeloma cell RPMI8226].

Abstract

This study was purposed to explore the caspase-independent apoptosis pathway in human multiple myeloma cell RPMI8226 induced by arsenic trioxide (As(2)O(3)). MTT method was used to analyze the proliferation inhibition rate; flow cytometry was used to detect the apoptosis rate; Western blot was used to determine the expressions of BCL-2 and Caspase-3 in RPMI8226 cells. The results showed that As(2)O(3) (0.1 - 20 µmol/L) significantly inhibited the proliferation of RPMI8226 (P < 0.05) in concentration- and time-dependent manner. Compared with the group treated with As(2)O(3) (10 µmol/L) alone, the apoptosis rate of zVAD-fmk (20 µmol/L) and As(2)O(3) combined treated group did not change. Compared with the group treated with As(2)O(3) (10 µmol/L) alone, zVAD-fmk (20 µmol/L) combined with As(2)O(3) (10 µmol/L) treatment group showed significant increase of expressions of Caspase-3 and BCL-2. It is concluded that As(2)O(3) can inhibit the proliferation of RPMI8226 cells. As(2)O(3) can induce apoptosis of RPMI8226 cells, and a caspase-independent process probably exist in As2O3-inducing RPMI8266 cells apoptosis.

• Authors: Jia Xie, Mei Zhang, Yan-Ping Song, Kai Hu, Jing-Jing Ren, Yun-Jie Zhang
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 20 Issue 1 Pg. 107-11 (Feb 2012) ISSN: 1009-2137 [Print] China
• PMID: 22391177 (Publication Type: Journal Article)

Chemical References

• Arsenicals
• Oxides
• Proto-Oncogene Proteins c-bcl-2
• CASP3 protein, human
• Caspase 3
• Arsenic Trioxide

Topics

• Apoptosis (drug effects)
• Arsenic Trioxide
• Arsenicals (pharmacology)
• Caspase 3 (metabolism)
• Cell Line, Tumor
• Humans
• Multiple Myeloma (metabolism, pathology)
• Oxides (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)