Abstract | BACKGROUND: METHODS: RESULTS: Qualitative RT-PCR revealed these proteases in ipsilateral and contralateral cortices. Dipeptidyl peptidase II and aminopeptidase N were up-regulated ipsilaterally from 6 h to 7 days post ischemia, whereas dipeptidyl peptidase 9 and cytosolic alanyl-aminopeptidase were transiently down-regulated at day 3. Dipeptidyl peptidase 8 and aminopeptidase N immunoreactivities were detected in cortical neurons of the contralateral hemisphere. At the same time point, dipeptidyl peptidase IV, 8 and aminopeptidase N were identified in activated microglia and macrophages in the ipsilateral cortex. Seven days post artery occlusion, dipeptidyl peptidase IV immunoreactivity was found in the perikarya of surviving cortical neurons of the ipsilateral hemisphere, whereas their nuclei were dipeptidyl peptidase 8- and amino peptidase N-positive. At the same time point, dipeptidyl peptidase IV, 8 and aminopeptidase N were targeted in astroglial cells. Total dipeptidyl peptidase IV, 8 and 9 activities remained constant in both hemispheres until day 3 post experimental ischemia, but were increased (+165%) in the ipsilateral cortex at day 7. In parallel, aminopeptidase N and cytosolic alanyl-aminopeptidase activities remained unchanged. CONCLUSIONS:
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Authors | Peter Röhnert, Werner Schmidt, Patrick Emmerlich, Alexander Goihl, Sabine Wrenger, Ute Bank, Karsten Nordhoff, Michael Täger, Siegfried Ansorge, Dirk Reinhold, Frank Striggow |
Journal | Journal of neuroinflammation
(J Neuroinflammation)
Vol. 9
Pg. 44
(Feb 28 2012)
ISSN: 1742-2094 [Electronic] England |
PMID | 22373413
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Enzyme Inhibitors
- Glial Fibrillary Acidic Protein
- Glycosphingolipids
- RNA, Messenger
- inositolphosphorylceramide
- CD13 Antigens
- Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
- Dpp8 protein, rat
- Dipeptidyl Peptidase 4
- Phosphopyruvate Hydratase
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Topics |
- Animals
- Brain Ischemia
(complications, drug therapy, enzymology)
- CD13 Antigens
(genetics, metabolism)
- Cerebral Infarction
(enzymology, etiology)
- Dipeptidyl Peptidase 4
(genetics, metabolism)
- Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
(genetics, metabolism)
- Disease Models, Animal
- Enzyme Inhibitors
(therapeutic use)
- Functional Laterality
- Gene Expression Regulation, Enzymologic
(physiology)
- Glial Fibrillary Acidic Protein
(metabolism)
- Glycosphingolipids
(therapeutic use)
- Male
- Phosphopyruvate Hydratase
(metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Time Factors
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