Oxytocin stimulates the cardiomyogenesis of embryonic stem cells and adult cardiac stem cells. We previously reported that
oxytocin has a promigratory effect on umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs). In this study, UCB-MSCs were cultured with
oxytocin and examined for their
therapeutic effect in an infarcted heart. UCB-MSCs were pretreated with 100 nM
oxytocin and cardiac markers were assessed by immunofluorescence staining. Next,
oxytocin-supplemented USC-MSCs (OT-USCs) were cocultured with
hypoxia/reoxygenated neonatal rat cardiomyocytes and cardiac markers and
dye transfer were then examined. For the in vivo study,
ischemia/reperfusion was induced in rats, and
phosphate-buffered saline (group 1), 1-day OT-USCs (group 2), or 7-day OT-USCs (group 3) were injected into the infarcted myocardium. Two weeks after injection, histological changes and cardiac function were examined. UCB-MSCs expressed
connexin 43 (Cnx43), cardiac
troponin I (cTnI), and α-sarcomeric actin (α-SA) after
oxytocin supplementation and coculture with cardiomyocytes. Functional gap junction formation was greater in group 3 than in groups 1 and 2. Cardiac
fibrosis and macrophage infiltration were lower in group 3 than in group 2. Restoration of Cnx43 expression was greater in group 3 than in group 2. Cnx43- and cTnI-positive OT-USCs in the peri-
infarct zone were observed in group 2 and more frequently in group 3. The ejection fraction (EF) was increased in groups 2 and 3 in 2 weeks. The improved EF was sustained for 4 weeks only in group 3. Our findings suggest that the supplementation of UCB-MSCs with
oxytocin can contribute to the cardiogenic potential for cardiac repair.