[Effect of chlorogenic acid on degranulation in mast cell RBL-2H3].

Abstract

OBJECTIVE:

To provide evidences for evaluating the role of chlorogenic acid (CA) on the adverse reaction of traditional Chinese medicine injection and promoting clinical rational usage of CA, the effect of CA and chlorogenic acid-HSA(CA-HSA) on the degranulation in mast cell RBL-2H3 were compared and the allergenic effect and its mechanism were investigated.

METHOD:

The unsensitized and sensitized RBL-2H3 cells were used. The releasing rate of histamine and beta-hexosaminidase was detected by colormetric assays. The degranulating rate was detected by neutral red staining and Annexin V positive cell rate was detected by flow cytometry.

RESULT:

CA and CA-HSA could not induce degranulation in unsensitized RBL-2H3 cells. CA and CA-HSA could significantly increase the release of histamine and beta-hexosaminidase, degranulating rate and Annexin V positive cell rate.

CONCLUSION:

CA has strong allergenicity after combination with serum proteins. As an active ingredient of Shuanghuanglian injection, CA is a kind of possible allergen which caused hypersensitivity reactions induced by Shuanghuanglian injection.

• Authors: Bo Peng, Rong He, Qihua Xu, Jie Gao, Yanli Lu, Jianrong Li
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 36 Issue 7 Pg. 912-7 (Apr 2011) ISSN: 1001-5302 [Print] China
• PMID: 21761734 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Chlorogenic Acid
• beta-N-Acetylhexosaminidases

Topics

• Animals
• Cell Degranulation (drug effects)
• Cell Line
• Chlorogenic Acid (adverse effects)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (adverse effects)
• Histamine Release (drug effects)
• Mast Cells (cytology, drug effects, metabolism)
• Rats
• beta-N-Acetylhexosaminidases (metabolism)