Requirement for store-operated calcium entry in sodium butyrate-induced apoptosis in human colon cancer cells.

Abstract

The SOCE (store-operated Ca2+ entry) pathway plays a key role in both normal cells and cancerous cells. However, its molecular mechanism remains a long-lasting puzzle of Ca2+ signalling. In this paper, we provide evidence that butyric acid, a dietary fibre-derived short-chain fatty acid, induces apoptosis of colon cancer cells via SOCE signalling networks. We found that sodium butyrate (NaB) induces Ca2+ release from endoplasmic reticulum, which in turn causes extracellular Ca2+ influx in HCT-116 cells. The Ca2+ release and influx are important, because the addition of chelators, EGTA or BAPTA/AM [1,2-bis-(o-aminophenoxy)ethane-N,N,N',N'-tetra-acetic acid tetrakis(acetoxymethyl ester)] respectively blocked NaB-induced apoptosis. Furthermore, down-regulation of STIM1 (stromal interaction molecule 1) by RNA interference or pharmacological blockade of the SOCC (store-operated Ca2+ channel) by 2-APB (2-aminoethoxydiphenyl borate) or SKF-96365 inhibited NaB-induced extracellular Ca2+ influx and apoptosis in HCT-116 cells. Thus we conclude that NaB triggers colon cancer cell apoptosis in an SOCE-dependent manner. This finding provides new insights into how butyric acid suppresses colon carcinogenesis.

• Authors: Suxia Sun, Wenjun Li, He Zhang, Longying Zha, Yong Xue, Xianbo Wu, Fei Zou
• Journal: Bioscience reports (Biosci Rep) Vol. 32 Issue 1 Pg. 83-90 (Feb 2012) ISSN: 1573-4935 [Electronic] England
• PMID: 21699495 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Boron Compounds
• Butyrates
• Chelating Agents
• Imidazoles
• Membrane Proteins
• Neoplasm Proteins
• STIM1 protein, human
• Stromal Interaction Molecule 1
• 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester
• Egtazic Acid
• 2-aminoethoxydiphenyl borate
• 1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
• Calcium

Topics

• Apoptosis (drug effects)
• Boron Compounds (pharmacology)
• Butyrates (pharmacology)
• Calcium (metabolism)
• Calcium Signaling
• Cell Line, Tumor
• Chelating Agents (pharmacology)
• Colonic Neoplasms (drug therapy, metabolism, pathology)
• Egtazic Acid (analogs & derivatives, pharmacology)
• Endoplasmic Reticulum (drug effects, metabolism)
• Humans
• Imidazoles (pharmacology)
• Membrane Proteins (genetics, metabolism)
• Neoplasm Proteins (genetics, metabolism)
• RNA Interference
• Stromal Interaction Molecule 1