Identification of peptide targets in neuromyelitis optica.

Abstract

Neuromyelitis optica (NMO) is an inflammatory demyelinating disease that predominantly affects the optic nerves and spinal cord. Recombinant antibodies (rAbs) generated from clonally expanded plasma cells in an NMO patient are specific to AQP4 and pathogenic. We screened phage-displayed peptide libraries with these rAbs, and identified 14 high affinity linear and conformational peptides. The linear peptides shared sequence homologies with NMO autoantigen AQP4 on the extracellular surface. Competitive inhibition ELISA and immunocytochemistry demonstrated that these peptides represent epitopes of NMO autoantigen AQP4. Peptide epitopes/mimotopes may have potential uses for disease prognosis, monitoring, and therapy.

• Authors: Xiaoli Yu, Miyoko Green, Don Gilden, Chiwah Lam, Katherine Bautista, Jeffrey L Bennett
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 236 Issue 1-2 Pg. 65-71 (Jul 2011) ISSN: 1872-8421 [Electronic] Netherlands
• PMID: 21621279 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Autoantibodies
• Immunoglobulin G
• Peptide Fragments
• Peptide Library
• SDC1 protein, human
• Syndecan-1

Topics

• Amino Acid Sequence
• Animals
• Autoantibodies (genetics, metabolism)
• Gene Targeting (methods)
• Humans
• Immunoglobulin G (genetics, metabolism)
• Molecular Sequence Data
• Neuromyelitis Optica (genetics, metabolism)
• Peptide Fragments (genetics, metabolism)
• Peptide Library
• Rats
• Rats, Inbred F344
• Syndecan-1 (genetics, metabolism)