Cloning of the flap endonuclease-1 gene in Bombyx mori and identification of an antiapoptotic function.

Abstract

The flap endonuclease-1 (FEN-1) gene is involved in DNA replication and repair, and it maintains genomic stability as well as the accuracy of DNA replication under normal growth conditions. However, FEN-1 also plays an important role in apoptosis and cancer development. We cloned the BmFEN-1 gene from Bombyx mori, which was 1343 bp in length and possessed an 1143 bp ORF (123-1266). It consists of seven introns and eight exons that encode a protein with 380 amino acids that has the typical XPG domain. The N-terminal motif is located at amino acids 95-105, and the proliferating cell nuclear antigen interaction motif is located at amino acids 337-344. RNA interference-mediated reduction of BmFEN-1 expression induced cell cycle arrest in S phase in BmE-SWU1 cells. These results suggest that BmFEN-1 can inhibit apoptosis and promote cell proliferation.

• Authors: Min-Hui Pan, Juan Du, Jin-Ye Zhang, Mao-Hua Huang, Ting Li, Hong-Juan Cui, Cheng Lu
• Journal: DNA and cell biology (DNA Cell Biol) Vol. 30 Issue 10 Pg. 763-70 (Oct 2011) ISSN: 1557-7430 [Electronic] United States
• PMID: 21612397 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Insect Proteins
• Flap Endonucleases

Topics

• Animals
• Apoptosis
• Base Sequence
• Bombyx (genetics, metabolism)
• Cell Cycle (genetics)
• Cell Proliferation
• Cell Survival
• Cells, Cultured
• Cloning, Molecular (methods)
• Databases, Genetic
• Exons
• Flap Endonucleases (chemistry, genetics, metabolism)
• Insect Proteins (chemistry, genetics, metabolism)
• Introns
• Larva (genetics, metabolism)
• Molecular Sequence Data
• Open Reading Frames
• Phylogeny
• Protein Structure, Tertiary
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid
• Transfection