Characterization of discriminatory urinary proteomic biomarkers for severe preeclampsia using SELDI-TOF mass spectrometry.

Abstract

OBJECTIVE:

To analyze the proteomic pattern in urine for distinguishing severe preeclampsia from mild preeclampsia and normotensive controls using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS).

STUDY DESIGN:

Urine samples were collected from women with severe preeclampsia (n=11 [sPE]), mild preeclampsia (n=7 [mPE]), and normotensive controls (n=8) and analyzed by SELDI-TOF-MS to identify discriminatory protein peaks in the sPE cohort. A scoring system was constructed--designated as Preeclampsia Proteomic Score of Urine (PPSU)--to differentiate sPE from mPE and normotensive controls.

RESULTS:

Four discriminatory protein peaks were identified (m/z ratio: 4155, 6044, 6663, and 7971), all of which were down-regulated in women with sPE. PPSU scores in women with sPE were significantly lower than that in both mPE and controls (sPE 0 [0-4] vs. mPE 3 [0-4] vs. controls 4 [2-4]; median [range]; P<0.05). PPSU<2 had a sensitivity of 90.9% and specificity of 93.3% in discriminating patients with sPE from mPE and controls.

CONCLUSION:

Proteomic analysis of urine can accurately distinguish sPE from mPE and normotensive controls.

• Authors: Seung Mi Lee, Joong Shin Park, Errol R Norwitz, Sun Min Kim, Byoung Jae Kim, Chan-Wook Park, Jong Kwan Jun, Hee Chul Syn
• Journal: Journal of perinatal medicine (J Perinat Med) Vol. 39 Issue 4 Pg. 391-6 (07 2011) ISSN: 1619-3997 [Electronic] Germany
• PMID: 21557676 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Proteome

Topics

• Adult
• Biomarkers (urine)
• Case-Control Studies
• Female
• Humans
• Pre-Eclampsia (diagnosis, urine)
• Pregnancy
• Proteome (analysis)
• Proteomics
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization