Urinary proteomics analysis for renal injury in hypertensive disorders of pregnancy with iTRAQ labeling and LC-MS/MS.

Abstract

PURPOSE:

As a noninvasive examination, urinary proteomics is a very useful tool to identify renal disease. The purpose of the present study was to find differential proteins among women with preeclampsia, gestational hypertension and normal pregnancy, and to screen potential biomarkers for the early diagnosis of preeclampsia.

EXPERIMENTAL DESIGN:

Urinary proteins were identified by iTRAQ labeling coupled with 2-D LC-MS/MS. The bioinformatics analysis was performed with the Metacore software and the International Protein Index (IPI) and the Gene Ontology (GO) Database. The differentially expressed proteins were verified by ELISA.

RESULTS:

362 nonredundant proteins were identified, 113 of which were expressed differentially between preeclampsia and normal pregnant group and 31 differential proteins among three groups. These differential proteins were associated with biological processes of blood coagulation, cell adhesion and differentiation, immune response and cytoskeleton development, etc. They interacted with each other in the network. The urinary angiotensinogen (AGT) was downregulated, which was consistent with the ELISA validation results.

CONCLUSIONS AND CLINICAL RELEVANCE:

The present study found a multitude of differential proteins that might provide a clue for investigating the mechanism of proteinuria development in preeclampsia. Low urinary angiotensinogen levels were useful for identifying preeclampsia.

• Authors: Guixiang Chen, Yang Zhang, Xiaohong Jin, Lihong Zhang, Yunjiao Zhou, Jianying Niu, Jing Chen, Yong Gu
• Journal: Proteomics. Clinical applications (Proteomics Clin Appl) Vol. 5 Issue 5-6 Pg. 300-10 (Jun 2011) ISSN: 1862-8354 [Electronic] Germany
• PMID: 21538910 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Angiotensinogen

Topics

• Acute Kidney Injury (complications, urine)
• Adult
• Angiotensinogen (urine)
• Case-Control Studies
• Chromatography, Liquid (methods)
• Computational Biology
• Enzyme-Linked Immunosorbent Assay
• Female
• Gene Expression Regulation
• Humans
• Hypertension, Pregnancy-Induced (urine)
• Pre-Eclampsia (urine)
• Pregnancy
• Proteinuria (urine)
• Proteomics (methods)
• Reproducibility of Results
• Staining and Labeling
• Tandem Mass Spectrometry (methods)