Telomerase-mediated apoptosis of chicken lymphoblastoid tumor cell line by lanthanum chloride.

Abstract

To investigate the biological effects of lanthanum on chicken lymphoid tumors, cultures of the MDCC-MSB1 chicken lymphoblastoid cell line were treated with 2.5 mM lanthanum chloride for 2 days at 12-h intervals. The apoptotic cells were detected using the TdT-mediated dUTP nick end labeling assay and flow cytometer analysis. The telomerase activity and the chTERT mRNA expression levels of the MDCC-MSB1 cells were examined. The results showed that MDCC-MSB1 cell apoptosis occurred after incubation for 12 to 48 h induced by 2.5 mM LaCl(3). Consistent with the apoptosis results, telomerase activities in LaCl(3)-treated cells significantly decreased (P < 0.05) compared with 0 h group. Furthermore, the transcription of chTERT gene in MDCC-MSB1 cell was significantly inhibited in LaCl(3) treatment group (P < 0.05). These results suggest that the decrease of the chTERT transcription and telomerase activity play an important role in the La-induced apoptosis in chicken lymphoid tumor.

• Authors: Ziwei Zhang, Jintao Wang, Jinlong Li, Shiwen Xu
• Journal: Biological trace element research (Biol Trace Elem Res) Vol. 144 Issue 1-3 Pg. 657-67 (Dec 2011) ISSN: 1559-0720 [Electronic] United States
• PMID: 21448564 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• RNA, Messenger
• lanthanum chloride
• Lanthanum
• Telomerase

Topics

• Animals
• Antineoplastic Agents
• Apoptosis (physiology)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Chickens (physiology)
• Down-Regulation (drug effects)
• Flow Cytometry
• In Situ Nick-End Labeling
• Lanthanum (pharmacology)
• Lymphoma (drug therapy, pathology)
• RNA, Messenger (biosynthesis, genetics)
• Real-Time Polymerase Chain Reaction
• Telomerase (physiology)