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Circulating endothelial progenitor cells are up-regulated in a mouse model of endometriosis.

Abstract
Endometriosis is a debilitating disease characterized by the growth of ectopic endometrial tissue. It is widely accepted that angiogenesis plays an integral part in the establishment and growth of endometriotic lesions. Recent data from a variety of angiogenesis-dependent diseases suggest a critical role of bone marrow-derived endothelial progenitor cells (EPCs) in neovascularization. In this study we examined the blood levels of EPCs and mature circulating endothelial cells in a mouse model of surgically induced endometriosis. Fluorescence-activated cell sorting analysis revealed elevated levels of EPCs in the blood of mice with endometriosis compared with control subject that underwent a sham operation. EPC concentrations positively correlated with the amount of endometriotic tissue and peaked 1 to 4 days after induction of disease. In a green fluorescent protein bone marrow transplant experiment we found green fluorescent protein-positive endothelial cells incorporated into endometriotic lesions but not eutopic endometrium, as revealed by flow cytometry and immunohistochemistry. Finally, treatment of endometriosis-bearing mice with the angiogenesis inhibitor Lodamin, an oral nontoxic formulation of TNP-470, significantly decreased EPC levels while suppressing lesion growth. Taken together, our data indicate an important role for bone marrow-derived endothelial cells in the pathogenesis of endometriosis and support the potential clinical use of anti-angiogenic therapy as a novel treatment modality for this disease.
AuthorsChristian M Becker, Paul Beaudry, Tae Funakoshi, Ofra Benny, Alexander Zaslavsky, David Zurakowski, Judah Folkman, Robert J D'Amato, Sandra Ryeom
JournalThe American journal of pathology (Am J Pathol) Vol. 178 Issue 4 Pg. 1782-91 (Apr 2011) ISSN: 1525-2191 [Electronic] United States
PMID21435458 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.
Chemical References
  • Angiogenesis Inhibitors
  • Cyclohexanes
  • Sesquiterpenes
  • Green Fluorescent Proteins
  • O-(Chloroacetylcarbamoyl)fumagillol
Topics
  • Administration, Oral
  • Angiogenesis Inhibitors (pharmacology)
  • Animals
  • Bone Marrow Transplantation
  • Cell Separation
  • Cyclohexanes (pharmacology)
  • Disease Models, Animal
  • Endometriosis (blood, pathology)
  • Endothelial Cells (cytology)
  • Female
  • Flow Cytometry
  • Green Fluorescent Proteins (metabolism)
  • Immunohistochemistry (methods)
  • Mice
  • Mice, Inbred C57BL
  • O-(Chloroacetylcarbamoyl)fumagillol
  • Sesquiterpenes (pharmacology)
  • Stem Cells (cytology)
  • Up-Regulation

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