Abstract | OBJECTIVE: To investigate the value of fluorescence in situ hybridization (FISH) detection of human telomerase RNA component ( hTERC) gene amplification in screening of cervical lesions. METHODS: A total of 146 post-thinPrep cytology test (TCT) samples were analyzed using FISH by two-color interphase probe targeting hTERC gene at chromosome 3q26 and the data were compared with the cytological and histological results. RESULTS: FISH analysis was successful in 120 cases (20 cases of normal and 100 abnormal cases by TCT). Gene amplification of hTERC by FISH had a positive correlation with the cytological (r = 0.465, P < 0.01) and histological grade results (r = 0.610, P < 0.01). Extra copies of hTERC were seen in 28.6% (6/21) of CINI, 61.1% (11/18) of CINII, 75.0% (18/24) of CINIII and 91.7%(22/24) of squamous cell carcinoma, respectively. None (0/13) of the inflammation cases showed hTERC amplification. The sensitivity and specificity for detecting high grade lesions by FISH were 77.3% (51/66) and 82.4% (28/34); and the positive and negative predictive values were 89.5% and 65.1%, respectively. The rate of hTERC gene gain in high grade lesions was significantly higher than that in the low grade lesions (χ(2) = 32.550, P < 0.01). Combined with the high copy numbers, the sensitivity for detecting high grade lesions was increased to 81.2%. CONCLUSIONS: Detection of hTERC gene amplification by FISH improves the screening efficiency of high-risk cervical epithelial lesions. The presence of high copy numbers of hTERC correlates with the presence of high grade cervical dysplasia.
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Authors | Li Gao, Yan Zhu, Su-ping Liu, Yan Gao, Ming-hua Zhu |
Journal | Zhonghua bing li xue za zhi = Chinese journal of pathology
(Zhonghua Bing Li Xue Za Zhi)
Vol. 40
Issue 2
Pg. 104-7
(Feb 2011)
ISSN: 0529-5807 [Print] China |
PMID | 21426806
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- telomerase RNA
- RNA
- Telomerase
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Topics |
- Adult
- Aged
- Carcinoma, Squamous Cell
(diagnosis, genetics, pathology)
- Female
- Gene Amplification
- Humans
- In Situ Hybridization, Fluorescence
- Middle Aged
- RNA
(genetics)
- Telomerase
(genetics)
- Uterine Cervical Neoplasms
(diagnosis, genetics, pathology)
- Uterine Cervicitis
(diagnosis, genetics, pathology)
- Young Adult
- Uterine Cervical Dysplasia
(diagnosis, genetics, pathology)
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