The effect of Allium sativum on ischemic preconditioning and ischemia reperfusion induced cardiac injury.

Abstract

In the present study, the effect of garlic (Allium sativum) extract on ischemic preconditioning and ischemia-reperfusion induced cardiac injury has been studied. Hearts from adult albino rats of Wistar strain were isolated and immediately mounted on Langendorff's apparatus for retrograde perfusion. After 15 minutes of stabilization, the hearts were subjected to four episodes of 5 min ischemia, interspersed with 5 min reperfusion (to complete the protocol of ischemic preconditioning), 30 min global ischemia, followed by 120 min of reperfusion. In the control and treated groups, respective interventions were given instead of ischemic preconditioning. The magnitude of cardiac injury was quantified by measuring Lactate Dehydrogenase and creatine kinase concentration in the coronary effluent and myocardial infarct size by macroscopic volume method. Our study demonstrates that garlic extract exaggerates the cardio protection offered by ischemic preconditioning and per se treatment with garlic extract also protects the myocardium against ischemia reperfusion induced cardiac injury.

• Authors: Rajbir Bhatti, Kushlinder Singh, M P S Ishar, Jatinder Singh
• Journal: Indian journal of pharmacology (Indian J Pharmacol) Vol. 40 Issue 6 Pg. 261-5 (Nov 2008) ISSN: 0253-7613 [Print] India
• PMID: 21279182 (Publication Type: Journal Article)