Abstract | BACKGROUND: METHODS: 21 clinical isolates of different patients with PD peritonitis secondary to Staphylococcus aureus were collected. They were analyzed for their antibiotic susceptibility in the planktonic form using the standard minimum inhibitory concentration (MIC) and in a biofilm using minimum biofilm eradication concentration (MBEC). Chi-square was used to compare the sensitivity results. ♢ RESULTS: The isolates were susceptible to all the antibiotics tested using MIC. Every antibiotic except gentamicin lost its efficacy when the bacteria were grown in a biofilm (p > 0.05). The change in susceptibility was statistically significant to a level of p < 0.001 for all antibiotics tested. ♢ DISCUSSION: In PD peritonitis that is long standing, recurrent, or not responsive to therapy, MBEC testing should be considered as a biofilm may be present. Gentamicin should be strongly considered over other agents for empiric gram-negative coverage as it may be providing synergy in the setting of Staphylococcus aureus. Also, the newer anti-staphylococcal drugs should be tested for their performance in a biofilm using the MBEC method.
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Authors | Louis P Girard, Howard Ceri, Allan P Gibb, Merle Olson, Farshad Sepandj |
Journal | Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis
(Perit Dial Int)
2010 Nov-Dec
Vol. 30
Issue 6
Pg. 652-6
ISSN: 1718-4304 [Electronic] United States |
PMID | 21148059
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Drug Combinations
- Gentamicins
- Clindamycin
- Ciprofloxacin
- Vancomycin
- Cefazolin
- Oxacillin
- Rifampin
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- Biofilms
(drug effects)
- Cefazolin
(pharmacology)
- Ciprofloxacin
(pharmacology)
- Clindamycin
(pharmacology)
- Drug Combinations
- Drug Resistance, Bacterial
(drug effects)
- Gentamicins
(pharmacology)
- Humans
- Microbial Sensitivity Tests
- Oxacillin
(pharmacology)
- Peritoneal Dialysis
(adverse effects)
- Peritonitis
(microbiology)
- Prosthesis-Related Infections
(microbiology)
- Rifampin
(pharmacology)
- Staphylococcal Infections
(microbiology)
- Staphylococcus aureus
(drug effects)
- Vancomycin
(pharmacology)
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