Patterns of gene expression in muscle and fat in tumor-bearing rats: effects of CRF2R agonist on cachexia.

Abstract

The hypothesis we tested was that administering corticotropin-releasing factor receptor agonists preserves muscle mass during cancer that is related to changes in tissue gene expression. cDNA microarrays were used to compare mRNAs from muscle and adipose tissues of non-treated and agonist-treated tumor-bearing rats. In muscle of non-tumor-bearing agonist-treated animals we observed decreased expression of genes associated with fatty acid uptake and esterification. In tumor-bearing animals, CRF2R agonist administration produced decreased mRNA content of the atrogene lipin-1. In white adipose tissue, agonist treatment of non-tumor-bearing animals induced genes typically related to muscle structure and function. The fact that this treatment decreased expression of atrogenes could have clinical application. In addition, agonist treatment changed the gene pattern of adipose tissue to render it similar to that of skeletal muscle; thus, treatment with this agonist alters the gene pattern to what could be called "muscularization of white adipose tissue."

• Authors: Josep M Argilés, Cibely Cristine Fontes-Oliveira, Gemma Fuster, Elisabet Ametller, Maite Figueras, Mireia Olivan, Francisco J Lopez-Soriano, Xiaoyan Qu, Jeffrey Demuth, Paula Stevens, Alex Varbanov, Feng Wang, Robert J Isfort, Sílvia Busquets
• Journal: Muscle & nerve (Muscle Nerve) Vol. 42 Issue 6 Pg. 936-49 (Dec 2010) ISSN: 1097-4598 [Electronic] United States
• PMID: 21104868 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CRF receptor type 2
• Lpin1 protein, rat
• Nuclear Proteins
• RNA, Messenger
• Receptors, Corticotropin-Releasing Hormone
• Corticotropin-Releasing Hormone

Topics

• Adipose Tissue (drug effects, metabolism)
• Analysis of Variance
• Animals
• Cachexia (genetics, metabolism)
• Corticotropin-Releasing Hormone (metabolism, pharmacology)
• Gene Expression
• Male
• Muscle, Skeletal (drug effects, metabolism)
• Neoplasm Transplantation
• Nuclear Proteins (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Wistar
• Receptors, Corticotropin-Releasing Hormone (agonists)
• Reverse Transcriptase Polymerase Chain Reaction
• Tissue Array Analysis