[HGPRT-defective Lewis lung carcinoma cell line and its biological characteristics].

Abstract

BACKGROUND:

To provide an HGPRT ( hypoxanthine guanidine phosphoribosyl transferase)-defective cell line for the establishment of lung cancer and dendritic cell( DC) fused vaccine.

METHODS:

The HGPRT-defective cell line was gradually induced by 8-AG ( 8-azaguanine) from the Lewis lung carcinoma cell line( L3-8) . Its biological characteristics and tumorigenicity were observed in vivo and in vitro.

RESULTS:

The HGPRT-defective cell line, AL9901, was obtained after one year of selective culture and identification. AL9901 cell line could grow steadily in 20mg/ L 8-AG, but not in HAT selective medium. The chromosome modal numbers of AL9901 and L3-8 cell line were 58 and 62 respectively, lung metastatic rates were 30%( 3/ 10) and 70%( 7/ 10) respectively, and their tumorigenic rates were both 100%( 10/ 10) .

CONCLUSIONS:

The HGPRT-defective cell line, AL9901, maintains the biolog ical characteristics and carcinogenicity of the L3-8 cell line. It can be used as a parental antigenic cell for the establishment of Lewis lung carcinoma-DC fused tumor vaccine.

• Authors: Y Lu, P Lin, J Zhang, X Wang, H Zhou, X Huang, Q Ning, Y Jiang, K Yang
• Journal: Zhongguo fei ai za zhi = Chinese journal of lung cancer (Zhongguo Fei Ai Za Zhi) Vol. 4 Issue 5 Pg. 324-6 (Oct 20 2001) ISSN: 1009-3419 [Print] China
• PMID: 21059307 (Publication Type: English Abstract, Journal Article)