Abstract | BACKGROUND: The antiatherogenic role of different HDL subclasses is still controversial. HDL particles of the same size can have different lipid contents in some physiopathological situations. However, little is known about the plasma lipid levels of HDL subclasses when they are separated by their hydrodynamic diameter. METHODS: RESULTS: Relative HDL size distribution was shifted to small particles in MS pediatric patients when determined per protein. In contrast, cholesterol plasma concentrations corresponding to the HDL2b, 2a, 3a, and 3b subclasses were decreased; triglycerides of HDL3b and 3c, as well as plasma phospholipids from HDL3c, were elevated in MS patients as compared to controls. The C-to-Ph ratio, considered as indicative of HDL composition, was similar among the 5 HDL subclasses in control subjects, whereas this ratio gradually decreased from large HDL2b to small HDL3c in the MS group. Cholesterol plasma concentrations of HDL subclasses correlated with the components of the MS. CONCLUSIONS:
Lipids of HDL subclasses provide more and accurate information than the relative HDL size distribution determined by protein staining, and may contribute to understand better HDL metabolism and the coronary risk associated to these lipoproteins.
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Authors | Cynthia García-Sánchez, Margarita Torres-Tamayo, Minerva Juárez-Meavepeña, Cristhel López-Osorio, Paola Toledo-Ibelles, Mariana Monter-Garrido, David Cruz-Robles, Elizabeth Carreón-Torres, Gilberto Vargas-Alarcón, Oscar Pérez-Méndez |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 412
Issue 3-4
Pg. 292-8
(Jan 30 2011)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 21036160
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2010 Elsevier B.V. All rights reserved. |
Chemical References |
- Enzymes
- Lipoproteins, HDL
- Phosphatidylcholines
- Triglycerides
- Cholesterol
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Topics |
- Case-Control Studies
- Child
- Cholesterol
(blood)
- Electrophoresis, Polyacrylamide Gel
(methods)
- Enzymes
(metabolism)
- Female
- Humans
- Lipoproteins, HDL
(blood, isolation & purification)
- Male
- Metabolic Syndrome
(blood)
- Phosphatidylcholines
(blood)
- Surface Properties
- Triglycerides
(blood)
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