Basophil CD203c levels are increased at baseline and can be used to monitor omalizumab treatment in subjects with nut allergy.

Abstract

RATIONALE:

Basophils contribute to anaphylaxis and allergies. We examined the utility of assessing basophil-associated surface antigens (CD11b/CD63/CD123/CD203c/CD294) in characterizing and monitoring subjects with nut allergy.

METHODS:

We used flow cytometry to analyze basophils at baseline (without any activation) and after ex vivo stimulation of whole blood by addition of nut or other allergens for 2, 10, and 30 min. We also evaluated whether basophil expression of CD11b/CD63/CD123/CD203c/CD294 was altered in subjects treated with anti-IgE monoclonal antibody (omalizumab) to reduce plasma levels of IgE.

RESULTS:

We demonstrate that basophil CD203c levels are increased at baseline in subjects with nut allergy compared to healthy controls (13 subjects in each group, p < 0.0001). Furthermore, we confirm that significantly increased expression of CD203c occurs on subject basophils when stimulated with the allergen to which the subject is sensitive and can be detected rapidly (10 min of stimulation, n = 11, p < 0.0008). In 5 subjects with severe peanut allergy, basophil CD203c expression following stimulation with peanut allergen was significantly decreased (p < 0.05) after 4 and 8 weeks of omalizumab treatment but returned toward pretreatment levels after treatment cessation.

CONCLUSIONS:

Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen. Both can be reduced by omalizumab therapy. These results highlight the potential of using basophil CD203c levels for baseline diagnosis and therapeutic monitoring in subjects with nut allergy.

• Authors: Yael Gernez, Rabindra Tirouvanziam, Grace Yu, Eliver E B Ghosn, Neha Reshamwala, Tammie Nguyen, Mindy Tsai, Stephen J Galli, Leonard A Herzenberg, Leonore A Herzenberg, Kari C Nadeau
• Journal: International archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 154 Issue 4 Pg. 318-27 ( 2011) ISSN: 1423-0097 [Electronic] Switzerland
• PMID: 20975283 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2010 S. Karger AG, Basel.

Chemical References

• Anti-Allergic Agents
• Antibodies, Anti-Idiotypic
• Antibodies, Monoclonal
• Antibodies, Monoclonal, Humanized
• Biomarkers
• ENPP3 protein, human
• Omalizumab
• Phosphoric Diester Hydrolases
• Pyrophosphatases

Topics

• Adolescent
• Adult
• Anti-Allergic Agents (therapeutic use)
• Antibodies, Anti-Idiotypic
• Antibodies, Monoclonal (therapeutic use)
• Antibodies, Monoclonal, Humanized
• Basophils (drug effects, immunology, metabolism)
• Biomarkers (analysis, blood)
• Cell Separation
• Child
• Child, Preschool
• Female
• Flow Cytometry
• Humans
• Infant
• Male
• Nut Hypersensitivity (drug therapy, immunology, metabolism)
• Omalizumab
• Phosphoric Diester Hydrolases (biosynthesis, drug effects)
• Pyrophosphatases (biosynthesis, drug effects)
• Sensitivity and Specificity
• Young Adult