Cytokine profiling in acute anterior cruciate ligament injury.

Abstract

PURPOSE:

To evaluate the presence and relative concentrations of cytokines, known to be involved in the inflammatory cascade, in acute anterior cruciate ligament (ACL) injury.

METHODS:

We evaluated an extensive cytokine profile in synovial fluid from 12 patients with acute ACL injury undergoing arthroscopy compared with 15 control subjects using a BioPlex assay (Bio-Rad Laboratories, Hercules, CA) to measure the concentration of 17 inflammatory cytokines.

RESULTS:

In patients with acute ACL injury compared with asymptomatic control subjects, the following cytokines were identified at significantly increased concentrations (P < .001, Mann-Whitney U test) compared with control samples: interleukin 6 (105 ± 72 v 0 ± 0 pg/ml), interferon γ (1,544 ± 608 v 9 ± 7.5 pg/ml), macrophage inflammatory protein 1β (16 ± 3.8 v 0.3 ± 0.2 pg/ml), and monocyte chemotactic protein 1 (35 ± 13 v 0.5 ± 0.4 pg/ml). There was no case of a cytokine exhibiting increased levels in asymptomatic compared with symptomatic knee samples.

CONCLUSIONS:

This investigation identified 4 specific cytokines (interleukin 6, interferon γ, monocyte chemotactic protein 1, and macrophage inflammatory protein 1β) out of a panel of 17 inflammatory molecules for which the levels were consistently elevated in the context of ACL injury compared with non-painful, non-acutely injured knees in a volunteer population.

LEVEL OF EVIDENCE:

Level IV, prognostic case series.

• Authors: Vanessa G Cuellar, Jason M Cuellar, S Raymond Golish, David C Yeomans, Gaetano J Scuderi
• Journal: Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association (Arthroscopy) Vol. 26 Issue 10 Pg. 1296-301 (Oct 2010) ISSN: 1526-3231 [Electronic] United States
• PMID: 20887928 (Publication Type: Journal Article)
• Copyright: Copyright © 2010 Arthroscopy Association of North America. Published by Elsevier Inc. All rights reserved.

Chemical References

• Chemokine CCL2
• Chemokine CCL4
• Cytokines
• Interleukin-8
• Interleukins
• Interferon-gamma

Topics

• Adult
• Anterior Cruciate Ligament Injuries
• Chemokine CCL2 (metabolism)
• Chemokine CCL4 (metabolism)
• Cytokines (metabolism)
• Humans
• Interferon-gamma (metabolism)
• Interleukin-8 (metabolism)
• Interleukins (metabolism)
• Multivariate Analysis
• Patient Selection
• Reference Values
• Synovial Fluid (metabolism)
• Young Adult