Expression of CD66 in non-Hodgkin lymphomas and multiple myeloma.

Abstract

OBJECTIVES:

⁹⁰Y-labeled anti-CD66b monoclonal antibody clone BW 250/183 was developed for treatment of tumors. The aim of the study was the analysis of CD66 expression in lymphoproliferative malignancies to expand the potential of anti-CD66-based therapy.

PATIENTS AND METHODS:

Bone marrow samples from 260 patients were examined for the expression of CD66 on tumor cells in 104 B-chronic lymphocytic leukemias (B-CLL), 28 mantle cell lymphomas (MCL), 22 follicular lymphomas (FCL), 15 marginal zone lymphomas (MZL), 12 lymphoplasmacytic lymphomas (LPL), 13 diffuse large B cell lymphomas (DLBCL), 4 T-non-Hodgkin lymphomas (T-NHL), 3 B-NHL not otherwise specified (B-NHL NOS), 3 B acute lymphoblastic leukemias (B-ALL), and in 56 multiple myelomas (MM) by flow cytometry.

RESULTS:

Positive (≥ 20%) expression of CD66abce clone Kat4c was detected in 76% of B-CLL and 76.8% of MM. The highest number of CD66abce-positive samples was in MCL and LPL (96.4% of 28 and 91.7% of 12 patients, respectively). Expression of CD66b clone BW 250/183 was examined in 114 of 260 samples. Positive expression was detected in 23.3% of B-CLL (6/35), 17.1% of MM (7/30), and 21.4% of MCL (3/14) samples.

CONCLUSION:

The expression of CD66b compared to CD66abce was lower in all measured samples. Use of radiolabeled anti-CD66b antibody for the treatment of lymphoproliferative diseases appears to have limited preclinical substantiation.

• Authors: Karban Josef, Mocikova Heidi, Pytlik Robert, Klener Pavel, Trneny Marek
• Journal: European journal of haematology (Eur J Haematol) Vol. 85 Issue 6 Pg. 496-501 (Dec 2010) ISSN: 1600-0609 [Electronic] England
• PMID: 20887387 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2010 John Wiley & Sons A/S.

Chemical References

• Antibodies, Monoclonal
• Antigens, CD
• CD66 antigens
• Cell Adhesion Molecules

Topics

• Antibodies, Monoclonal (chemistry, therapeutic use)
• Antigens, CD (biosynthesis)
• Bone Marrow (metabolism, pathology)
• Cell Adhesion Molecules (biosynthesis)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lymphoma, Non-Hodgkin (metabolism, pathology, radiotherapy)
• Male
• Multiple Myeloma (metabolism, pathology, radiotherapy)
• Radioimmunotherapy (methods)