Identification of a tumour associated antigen in lung cancer patients with asbestos exposure.

Abstract

BACKGROUND:

This study analysed the humoral immune response in asbestos exposed lung cancer patients to identify new surrogate markers of the carcinogenic risk in populations exposed to asbestos.

METHODS AND RESULTS:

A serological analysis identified five distinct antigens reactive with IgG derived from a lung cancer patient with high asbestos exposure. In one of the isolated antigens, quantitative RT-PCR indicated that annexin A2 (AnxA2) was overexpressed in lung cancer tissues and normal lung from patients with high asbestos exposure. Antibody against AnxA2 was detected in 9/15 (60%) of lung cancer patients with high asbestos exposure; however, in only 1/12 (8%) of lung cancer patients with low asbestos exposure. AnxA2 was also overexpressed in malignant mesothelioma cells, and the antibody was also positive in 8/15 (53%) of patients with malignant mesothelioma.

CONCLUSION:

The antibody titer against AnxA2 may be a potentially useful new diagnostic surrogate marker for asbestos-related lung cancer and malignant mesothelioma.

• Authors: Manabu Yasuda, Takeshi Hanagiri, Yoshiki Shigematsu, Takamitsu Onitsuka, Kouji Kuroda, Tetsurou Baba, Makiko Mizukami, Yoshinobu Ichiki, Hidetaka Uramoto, Mitsuhiro Takenoyama, Kosei Yasumoto
• Journal: Anticancer research (Anticancer Res) Vol. 30 Issue 7 Pg. 2631-9 (Jul 2010) ISSN: 1791-7530 [Electronic] Greece
• PMID: 20682992 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ANXA2 protein, human
• Annexin A2
• Antibodies, Neoplasm
• Antigens, Neoplasm
• Immunoglobulin G
• Interleukin-6
• Asbestos

Topics

• Aged
• Aged, 80 and over
• Annexin A2 (biosynthesis, immunology)
• Antibodies, Neoplasm (immunology)
• Antigens, Neoplasm (immunology)
• Asbestos (immunology, poisoning)
• Enzyme-Linked Immunosorbent Assay
• Humans
• Immunity, Humoral (immunology)
• Immunoglobulin G (immunology)
• Interleukin-6 (blood, immunology)
• Lung Neoplasms (etiology, immunology)
• Male
• Mesothelioma (etiology, immunology)
• Reverse Transcriptase Polymerase Chain Reaction