Abstract |
The zinc finger transcription factor ZNF24 is a relatively uncharacterized Krüppel-like transcription factor, displaying a suppressive effect on the transcription. ZNF24 is involved in negative regulation of vascular endothelial growth factor ( VEGF) and may represent a novel repressor of VEGF transcription. In a previous study, our analysis reveals that ZNF24 is a pleiotropic factor that has a role in hematopoiesis, brain development and cancers. Activation of PDGFR-beta is critically involved into various cell functions including recruitment of stromal cells and VEGF induction in tumor and perivascular cells. Here, overexpression of ZNF24 resulted in a significant down-regulation of PDGFR-beta, whereas silencing of ZNF24 with small interfering RNA led to increased PDGFR-beta expression. Overexpression of ZNF24 resulted in a significant decrease in PDGFR-beta promoter activity. These data suggest that ZNF24 is involved in negative regulation of PDGFR-beta and may represent a novel repressor of PDGFR-beta transcription.
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Authors | Jianzhong Li, Xia Chen, Ying Liu, Li Ding, Lei Qiu, Zhenlin Hu, Junping Zhang |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 397
Issue 2
Pg. 318-22
(Jun 25 2010)
ISSN: 1090-2104 [Electronic] United States |
PMID | 20510677
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Crown Copyright (c) 2010. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Kruppel-Like Transcription Factors
- RNA, Small Interfering
- Repressor Proteins
- ZNF24 protein, human
- Receptor, Platelet-Derived Growth Factor beta
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Topics |
- Cell Line
- Cell Line, Tumor
- Down-Regulation
- Gene Expression Regulation
- Gene Knockdown Techniques
- Humans
- Kruppel-Like Transcription Factors
(genetics, metabolism)
- Promoter Regions, Genetic
- RNA, Small Interfering
(genetics)
- Receptor, Platelet-Derived Growth Factor beta
(genetics)
- Repressor Proteins
(genetics, metabolism)
- Transcription, Genetic
- Up-Regulation
- Zinc Fingers
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