Abstract | OBJECTIVE: METHOD: Adult Roman hens were randomly divided into three groups, control, TOCP and PMSF + TOCP. Birds in PMSF + TOCP set were pretreated with PMSF, 24 hours later, hens in both TOCP group and PMSF + TOCP group were administrated with TOCP at a single dosage of 750 mg/kg. Then all animals were sacrificed on the corresponding time-points of 1, 5, 10, and 21 days respectively after dosing of 750 mg/kg TOCP. The spinal cords were dissected, homogenized, and centrifuged at 100,000 x g. The levels of high molecular neurofilament (NF-H), medium molecular neurofilament (NF-M) and low molecular neurofilament (NF-L) in both pellet and supernatant fractions of spinal cords were determined by SDS-PAGE and Western-blotting. RESULTS: The hens in TOCP group showed paralysis gait at the end of 21-day experimental period. The levels of NFs proteins in spinal cords changed obviously. Compared with control, the NFs in pellet showed a dramatic decrease on day 10 and then followed by a recovery. In the supernatant, the NFs proteins showed similar changes, which decreased significantly on day 10 and almost recovered control on day 21. Such as, NF-L, NF-M and NF-H decreased by 51%, 86% and 38% on day 10. The OPIDN signs were not observed in PMSF + TOCP group, and imbalances of NFs were obviously alleviated. Compared with control, only NF-M in pellet increased by 21% (P < 0.05) on day 21, others remained no changes; The levels of NF-H and NF-M in supernatant respectively increased by 19% and 35% on day 21, others were no significant statistical differences. CONCLUSION:
TOCP may induce imbalance of NFs levels in progress of OPIDN, and PMSF pretreatment may protect animals from OPIDN by reducing above changes, which may explain that TOCP-induced imbalance of NFs may be connected with the occurrence and development of OPIDN.
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Authors | Dan-Dan Dou, Fu-Yong Song, Xing Xin, Sheng Zhao, Tao Zeng, Cui-Li Zhang, Xiu-Lan Zhao, Ke-Qin Xie |
Journal | Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
(Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi)
Vol. 28
Issue 4
Pg. 250-4
(Apr 2010)
ISSN: 1001-9391 [Print] China |
PMID | 20465948
(Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Neurofilament Proteins
- Protein Subunits
- Tritolyl Phosphates
- Phenylmethylsulfonyl Fluoride
- tri-o-cresyl phosphate
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Topics |
- Animals
- Chickens
- Female
- Neurofilament Proteins
(drug effects)
- Phenylmethylsulfonyl Fluoride
(pharmacology)
- Protein Subunits
(drug effects)
- Spinal Cord
(drug effects, metabolism, pathology)
- Tritolyl Phosphates
(toxicity)
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