Abstract | BACKGROUND: Animal experiments have suggested that the laparoscopic peritoneal environment is hypoxic. This study aimed to investigate whether peritoneal tissue is hypoxic on a cellular level during a carbon dioxide (CO(2)) pneumoperitoneum at different intraperitoneal pressures (IPPs) and to determine the short-term effects of surgical injury on the hypoxia status of peritoneal tissue in the injured peritoneum and the distant noninjured peritoneum at cellular and molecular levels. METHODS: Experiment 1: Mice were divided into five groups according to the following treatments: anesthesia alone, laparotomy, and CO(2) pneumoperitoneum at IPPs of 2, 8, or 15 mmHg. Over the course of each experiment, the peritoneal tissue- oxygen tension (PitO(2)) was continuously monitored. Experiment 2: On the first day, the mice were divided into three groups according to the following treatments: CO(2) pneumoperitoneum at an IPP of either 2 or 8 mmHg or laparotomy. The bilateral caudal epigastric arteries and uterine horns then were coagulated using a bipolar cautery device. On day 7, peritoneal tissue samples were collected for real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. In both experiments, pimonidazole hydrochloride was used to detect tissue hypoxia at a cellular level. RESULTS: Experiment 1: Peritoneal hypoxia at both tissue and cellular levels was detected only in the groups treated with an IPP of 15 mmHg (PitO(2): 5.2 ± 1.0 mmHg, mean ± SEM). Experiment 2: The percentage of pimonidazole immunostained mesothelial and stromal cells from the distant noninjured peritoneum was significantly higher in the group treated with an IPP of 8 mmHg than in the other groups. Hypoxia-inducible factor 1 alpha subunit mRNA expression in the distant noninjured peritoneum of the group treated with an IPP of 8 mmHg was significantly higher than in the control group ( anesthesia alone). CONCLUSION: The CO(2) pneumoperitoneum itself did not cause peritoneal hypoxia at either a tissue or a cellular level in a mouse model when a low IPP was used.
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Authors | Sachiko Matsuzaki, Kris Jardon, Elodie Maleysson, Francis D'Arpiany, Michel Canis, Jean-Etienne Bazin, Gérard Mage |
Journal | Surgical endoscopy
(Surg Endosc)
Vol. 24
Issue 11
Pg. 2871-80
(Nov 2010)
ISSN: 1432-2218 [Electronic] Germany |
PMID | 20419320
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hypoxia-Inducible Factor 1
- Nitroimidazoles
- Plant Proteins
- RT-TIP protein, Nicotiana tabacum
- Carbon Dioxide
- pimonidazole
- Oxygen
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Topics |
- Animals
- Carbon Dioxide
(administration & dosage)
- Cell Hypoxia
- Female
- Hypoxia-Inducible Factor 1
(metabolism)
- Immunohistochemistry
- Laparotomy
- Mice
- Mice, Inbred C57BL
- Nitroimidazoles
(metabolism)
- Oxygen
(metabolism)
- Peritoneal Cavity
(physiology)
- Peritoneum
(metabolism, surgery)
- Plant Proteins
- Pneumoperitoneum, Artificial
- Pressure
- Respiration, Artificial
- Tissue Adhesions
(physiopathology)
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