Abstract |
In vivo studies suggest that the phenolic component contributes to the anti-inflammatory and antiatherosclerotic actions of olive oil; however, the effects in circulating cells are not fully characterized. Monocytes play a key role in inflammation-based diseases by expressing several molecules, including metalloproteinases ( MMPs). In the present study, we investigated the effects of olive oil phenolic extract and individual compounds on MMP-9 in THP-1 cells, a human monocyte-like cell line. Olive oil extract prevented the stimulation of MMP-9 expression and secretion in tumor necrosis factor alpha-treated THP-1 cells. Oleuropein aglycone, a typical olive oil phenol, was active at concentrations found in the extract, although other compounds probably contribute to the biological activity. We also found that the effect of the extract and individual compounds on MMP-9 is due to impaired nuclear factor-kappaB signaling. Our findings provide further evidence on the mechanisms by which olive oil reduces the inflammatory burden associated with disorders, such as atherosclerosis.
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Authors | Mario Dell'Agli, Rossana Fagnani, Germana V Galli, Omar Maschi, Federica Gilardi, Stefano Bellosta, Maurizio Crestani, Enrica Bosisio, Emma De Fabiani, Donatella Caruso |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 58
Issue 4
Pg. 2246-52
(Feb 24 2010)
ISSN: 1520-5118 [Electronic] United States |
PMID | 20102205
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA Primers
- NF-kappa B
- Olive Oil
- Phenols
- Plant Oils
- Tumor Necrosis Factor-alpha
- Matrix Metalloproteinase 9
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Topics |
- Cell Line, Tumor
- DNA Primers
- Gene Expression Regulation, Enzymologic
(drug effects)
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Leukemia, Myeloid
- Matrix Metalloproteinase 9
(genetics)
- NF-kappa B
(genetics, physiology)
- Olive Oil
- Phenols
(isolation & purification, pharmacology)
- Plant Oils
(chemistry)
- Promoter Regions, Genetic
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
(drug effects, physiology)
- Transcription, Genetic
(drug effects)
- Tumor Necrosis Factor-alpha
(pharmacology)
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