Porphyromonas gingivalis antigens and interleukin-6 stimulate the production of monocyte chemoattractant protein-1 via the upregulation of early growth response-1 transcription in human coronary artery endothelial cells.

Abstract

BACKGROUND:

Individuals with periodontitis have elevated serum levels of IL-6 and C-reactive protein and have been reported to have a significantly increased risk of developing cardiovascular disease. The transcription factor early growth response factor 1 (Egr-1) has been shown to play an important role in the development and progression of atherosclerosis. However, it is not known whether periodontal infection affects the expression of Egr-1 and subsequent endothelial cells expression of monocyte chemoattractant protein (MCP)-1, a key molecule of leukocyte chemoattraction into vessels.

METHODS:

Human coronary artery endothelial cells (HCAECs) were stimulated with either sonicated extracts from Porphyromonas gingivalis strains 381 or SU63, or a combination of IL-6 and soluble IL-6 receptor (IL-6/sIL-6R). The expression of Egr-1, and subsequently MCP-1, was then analyzed. The role of Egr-1 on MCP-1 expression was analyzed by siRNA transfection.

RESULTS:

Both P. gingivalis antigens and IL-6/sIL-6R stimulations upregulated the expression of Egr-1, with a more robust effect by IL-6/sIL-6R. Increased expression of Egr-1 coincided with MCP-1 production, and Egr-1 downregulation by siRNA suppressed this effect.

CONCLUSION:

These results clearly suggest that periodontal infection has the potential to affect HCAECs and hence contribute to the development of subsequent atherosclerosis.

• Authors: Tomoki Maekawa, Naoki Takahashi, Tomoyuki Honda, Daisuke Yonezawa, Hirotaka Miyashita, Takafumi Okui, Koichi Tabeta, Kazuhisa Yamazaki
• Journal: Journal of vascular research (J Vasc Res) Vol. 47 Issue 4 Pg. 346-54 ( 2010) ISSN: 1423-0135 [Electronic] Switzerland
• PMID: 20016208 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 S. Karger AG, Basel.

Chemical References

• Antigens, Bacterial
• CCL2 protein, human
• Chemokine CCL2
• EGR1 protein, human
• Early Growth Response Protein 1
• IL6 protein, human
• IL6R protein, human
• Interleukin-6
• RNA, Messenger
• Receptors, Interleukin-6
• Recombinant Proteins

Topics

• Antigens, Bacterial (immunology)
• Cells, Cultured
• Chemokine CCL2 (genetics, metabolism)
• Coronary Vessels (immunology, metabolism)
• Early Growth Response Protein 1 (genetics, metabolism)
• Endothelial Cells (immunology, metabolism)
• Humans
• Interleukin-6 (metabolism)
• Porphyromonas gingivalis (immunology)
• RNA Interference
• RNA, Messenger (metabolism)
• Receptors, Interleukin-6 (metabolism)
• Recombinant Proteins (metabolism)
• Up-Regulation