Fluorescence in situ hybridization analysis using PAX8- and PPARG-specific probes reveals the presence of PAX8-PPARG translocation and 3p25 aneusomy in follicular thyroid neoplasms.

Abstract

At the present time, the differentiation between follicular thyroid carcinoma (FTC) and adenoma can be made only postoperatively and is based on the presence of capsular or vascular invasion. The ability to differentiate preoperatively between the malignant and benign forms of follicular thyroid tumors assumes greater importance in any clinical setting. The PAX8-PPARG translocation has been reported to occur in the majority of FTC. In this study, a group of 60 follicular thyroid neoplasms [18 FTC, 1 Hurthle cell carcinoma (HCC), 24 follicular thyroid adenomas (FTA), 5 Hurthle cell adenomas (HCA), and 12 follicular variants of papillary thyroid carcinomas (FV-PTC)] were analyzed to determine the prevalence of the PAX8-PPARG translocation by fluorescence in situ hybridization. The PAX8-PPARG translocation was detected in 2/18 FTC (11.1%). In addition, 2/18 (11.1%) FTC and 1/5 (20%) HCA showed 3p25 aneusomy only. The frequency of the translocation detected in the study was lower compared to the earlier studies conducted in Western countries. This might be attributed to the ethnic background and geographic location. Detection of either the PAX8-PPARG translocation or the 3p25 aneusomy in FTC indicates that these are independent genetic events. It is hereby concluded that 3p25 aneusomy or PAX8-PPARG translocation may play an important role in the molecular pathogenesis of follicular thyroid tumors.

• Authors: Wai Kit Chia, Noor Akmal Sharifah, Rahayu Md Zin Reena, Zakaria Zubaidah, Ching Huat Clarence-Ko, Muhammad Rohaizak, Ibrahim Naqiyah, Das Srijit, Abdullah Nor Hisham, Arbi Asmiati, Md Kaslan Rafie
• Journal: Cancer genetics and cytogenetics (Cancer Genet Cytogenet) Vol. 196 Issue 1 Pg. 7-13 (Jan 01 2010) ISSN: 1873-4456 [Electronic] United States
• PMID: 19963130 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Molecular Probes
• PAX8 Transcription Factor
• PAX8 protein, human
• PPAR gamma
• Paired Box Transcription Factors

Topics

• Chromosome Aberrations
• Chromosomes, Artificial, Bacterial
• Chromosomes, Human, Pair 3
• Humans
• In Situ Hybridization, Fluorescence
• Molecular Probes
• PAX8 Transcription Factor
• PPAR gamma (genetics)
• Paired Box Transcription Factors (genetics)
• Thyroid Neoplasms (genetics)
• Translocation, Genetic