[Possible mechanisms of peripheral elevated CD8+ IL-17+ T cells in rheumatoid arthritis].

Abstract

OBJECTIVE:

To identify interleukin-17 (IL-17 )-producing CD8 positive T cells from patients with rheumatoid arthritis (RA), and investigate its cytokines as well as their correlations.

METHODS:

Peripheral blood got from 39 RA patients and 40 healthy donors. Flow cytometry was used to analyze the subsets of T cells in peripheral blood from these 39 RA patients and 27 healthy donors. 11-17, IL-6, and IL-23 levels in sera of all these people were tested by ELISA.

RESULTS:

The median of CD8 IL-17+ T cells from RA patients was 2.7% (95% confidence internal was 1.55%-3.74%); the median of these cells from healthy controls was 1.61% (95% confidence internal was 1.25%-2.61%). CD8+ IL-17+ T cells elevated significantly in RA(P < 0.05). As the production of CD8+ IL-17+ T cells, the IL-17 levels in sera of RA patients and healthy controls were (429 +/- 502) ng/L and (13 +/- 30) ng/L respectively. The IL-17 level in RA is higher than that in healthy people (P < 0.01). Meanwhile, to constitute CD8+ IL-17+ T cell polarizing condition, IL-23 and IL-6 in sera of RA were (157.83 +/- 27.07) ng/L and (32.67 +/- 34.50) x ng/L individually. These two cytokines in healthy controls were (21.97 +/- 3.52) ng/L and (1.82 +/- 1.51) ng/L severally. IL-6 and IL-23 increased clearly in peripheral blood of RA patients as compared with healthy donors (P < 0.01).

CONCLUSIONS:

This study suggested that elevated CD8+ IL-17+ T cells which involved in IL-17 secretion may depend on increased IL-6 and IL-23 in peripheral blood of rheumatoid arthritis.

• Authors: Tian Wang, Yi Zhao, Xu Liu, Xia Liu, Ru Li, Li-min Ren, Hua Ye, Zhan-guo Li
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 89 Issue 27 Pg. 1885-8 (Jul 21 2009) ISSN: 0376-2491 [Print] China
• PMID: 19953909 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-17
• Interleukin-23
• Interleukin-6

Topics

• Adult
• Aged
• Arthritis, Rheumatoid (blood, immunology)
• CD8-Positive T-Lymphocytes (metabolism)
• Case-Control Studies
• Female
• Flow Cytometry
• Humans
• Interleukin-17 (blood)
• Interleukin-23 (blood)
• Interleukin-6 (blood)
• Male
• Middle Aged