Plasma granzyme B as a predicting factor of coronary artery disease--clinical significance in patients with chronic renal failure.

Abstract

OBJECTIVES:

To elucidate the role of granzyme B in coronary artery disease (CAD) in patients with chronic kidney disease (CKD). We hypothesized that granzyme B plays an important role in the formation of coronary artery lesions in patients with CKD.

PATIENTS AND METHODS:

We studied 141 patients (116 men and 25 women; mean age, 64.2±9.6 years) and 16 control subjects. Diagnosis of CAD was confirmed by selective coronary angiography. CKD was defined as a sustained decrease in the estimated glomerular filtration (eGFR) rate less than 60 mL/min/1.73 m(2) over 3 months. We assigned patients to three groups: CAD without CKD (CAD group, n=46), CKD without CAD (CKD group, n=18), and CAD with CKD (CAD/CKD group, n=77). Plasma granzyme B was measured by enzyme-linked immunosorbent assay. Factors contributing to the severity of CAD were analyzed by multiple regression analysis in patients with CAD.

RESULTS:

Plasma levels of high-sensitivity CRP (hs-CRP) and granzyme B in the CAD/CKD group were significantly higher than in other groups. A significant positive correlation was observed between plasma hs-CRP and granzyme B levels. A significant negative correlation was observed between eGFR and granzyme B levels. Multiple regression analysis revealed that granzyme B and hs-CRP levels were independent predicting variables of the number of stenoses in major coronary arteries.

CONCLUSIONS:

These results indicate that granzyme B might be a novel risk factor for the formation of coronary atherosclerosis by inducing apoptosis of vascular tissues in patients with CKD.

• Authors: Tomokazu Ikemoto, Yukihiro Hojo, Hideyuki Kondo, Nozomu Takahashi, Masahiro Hirose, Yoshioki Nishimura, Takaaki Katsuki, Kazuyuki Shimada
• Journal: Journal of cardiology (J Cardiol) Vol. 54 Issue 3 Pg. 409-15 (Dec 2009) ISSN: 1876-4738 [Electronic] Netherlands
• PMID: 19944316 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• C-Reactive Protein
• Granzymes

Topics

• Aged
• Apoptosis
• Biomarkers (blood)
• C-Reactive Protein (analysis)
• Coronary Artery Disease (diagnosis, etiology, pathology)
• Coronary Vessels (cytology, pathology)
• Female
• Glomerular Filtration Rate
• Granzymes (blood, physiology)
• Humans
• Kidney Failure, Chronic (complications, diagnosis, pathology)
• Male
• Middle Aged
• Predictive Value of Tests
• Regression Analysis
• Risk Factors
• Severity of Illness Index